Application evaluation on peripheral blood nucleated red blood cell determination by SYSMEX XN-3000 automatic blood cell analyzer

doi:10.3969/j.issn.1673-8640.2015.04.015

Abstract

Abstract:

Objective To evaluate the methodology characteristics and clinical application significance of SYSMEX XN-3000 automatic blood cell analyzer(XN-3000) for the determination of peripheral blood nucleated red blood cell (NRBC). MethodsBy XN-3000 and manulal microscopy, 96 blood specimens were determined, and the correlation was analyzed by SPSS 11.0 statistical software. ResultsBy XN-3000, the sensitivity was 98.41%, the specificity was 93.94%, the false positive rate was 3.13%, and the false negative rate was 3.13%. The repeatability was good, the low coefficient of variation (CV) was 6.5%, and the middle and high CV were <5%. There was a good correlation for NRBC and manual microscopy (r²>0.867). Comparison of 2 methods showed no statistical significance (P=0.019). ConclusionsUsing XN-3000 for peripheral blood NRBC is reliable, simple and rapid with high accuracy, which can take the place of microscopy, and has certain clinical application significance.

Key words: Nucleated red blood cell, Automatic blood cell analyzer, Blood cell count

CLC Number:

R446.11

ZHAO Yuan, HU Enliang, ZHENG Tian, WANG Yan, ZHENG Shanluan.. Application evaluation on peripheral blood nucleated red blood cell determination by SYSMEX XN-3000 automatic blood cell analyzer[J]. Laboratory Medicine, 2015, 30(4): 356-358.

Figures/Tables 3

References 8

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标本类型	NRBC(%)	CV(%)
高值	134.50±1.45	1.1
中值	12.47±0.40	3.2
低值	3.10±0.20	6.5

标本类型	NRBC(%)	CV(%)
高值	134.50±1.45	1.1
中值	12.47±0.40	3.2
低值	3.10±0.20	6.5

XN-3000	显微镜镜检法		合计
XN-3000	阳性		阴性
阳性	62	2	64
阴性	1	31	32
合计	63	33	96

XN-3000	显微镜镜检法		合计
XN-3000	阳性		阴性
阳性	62	2	64
阴性	1	31	32
合计	63	33	96

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