Abstract:

Objective　To establish a method for determining the serum concentrations of 11 free fatty acids(FFA) in patients with coronary disease. Methods　The serum concentrations of 11 FFA in patients with coronary disease were determined by gas chromatography-mass spectrometry(GC/MS) combined with derivation reaction and N,O-bis(trimethylsilyl) trifluoroacetamide(BSTFA) as derivative reagent. Results　Chromatographic peaks of 11 FFA were separated completely,and all peak shapes were good. The interference of serum impurities on the determination did not occur. The curves showed good linearity. The recoveries of 11 FFA were>75%,respectively. The relative standard deviations(RSD) of intra-day and inter-day were<10%,respectively. Conclusions　The method is sensitive,accurate,easy to operate and time-saving. It provides referential basis to the determination of serum FFA in coronary disease.

Key words: Free fatty acid, Derivation, Gas chromatography-mass spectrometry, Coronary disease