Abstract:

Objective To establish a simple and rapid method for the determination of small dense low-density lipoprotein(sdLDL) by utilizing the principle of precipitation. Methods The final and most appropriate precipitation reagents were identified through comparing the separation effect of different types and different concentrations of precipitation reagents for separating serum lipoproteins. The precision, linear range and recovery rate of chemistry precipitation assay were also analyzed. Results Finally, 140 U/mL of heparin and 90 mmol/L of Mg²⁺ as the precipitation reagents were used to determine the concentrations of serum sdLDL cholesterol(sdLDL-C). The chemistry precipitation assay was linear at 0.14-1.44 mmol/L sdLDL-C, and the regression equation was Y＝1.025X－0.197, r＝0.988. The within-run coefficients of variation(CV) of high and low sdLDL-C concentration specimens were 2.83% and 3.19%, respectively, and the between-run CV were 5.49% and 6.13%. The recovery rate was 83.81%. Conclusions The chemistry precipitation assay for the determination of sdLDL has the advantages of less serum consumption, simple operation, low cost and so on. It is more suitable for clinical laboratory to determine serum sdLDL-C.

Key words: Small dense low-density lipoprotein, Precipitation assay, Chemical method