Study on the correlation of CR1 gene polymorphism with erythrocyte CR1 expression and its adhesive activity in patients with type 2 diabetes mellitus

Abstract

Abstract: Objective　To study on the correlation of complement receptor type 1 (CR1) gene polymorphism with erythrocyte CR1 expression and its adhesive activity in patients with type 2 diabetes mellitus (T2DM). Methods　A total of 132 patients with T2DM were determined for the CR1 gene polymorphism by restriction incision enzyme Hind Ⅲ polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)，and enzyme-linked immunosorbent assay (ELISA) was performed to detect the expression of CR1 on erythrocyte and its adhesive activity.Meanwhile，124 healthy subjects were enrolled as controls. The results were analyzed statistically. Results　The expression of CR1 on erythrocyte and its adhesive activity in patients with T2DM were lower than those in healthy controls (P<0.01). The CR1 genotype distribution frequencies from high to low were type HH，type HL and type LL by turns. The allele L frequency of CR1 gene in T2DM patients (14.8%) was higher than that in healthy controls (11.3%)，and there was no statistical significance in CR1 genotype frequency and allele frequency between the 2 groups (P>0.05). The expression of CR1 and adhesive activity of type HH and type HL had no statistical significance (P>0.05). The expression of CR1 and adhesive activity of type HH and type HL in T2DM patients were significantly lower than those in healthy controls (P<0.05). Conclusions　The genotype and allele frequencies in patients with T2DM have no statistical difference with those in healthy controls，but the expression of CR1 on erythrocyte and the adhesive activity in patients with T2DM decrease significantly. It indicates that the decrease of erythrocyte CR1 adhesive activity may be attribute to the occurrence of T2DM.

Key words: Complement receptor 1, Gene polymorphism, Erythrocyte, Adhesive activity, Type 2 diabetes mellitus

AN Xin;GAO Lichang;WANG Dongmei;ZOU Jimin;LI Chao;YUAN Baojun. Study on the correlation of CR1 gene polymorphism with erythrocyte CR1 expression and its adhesive activity in patients with type 2 diabetes mellitus[J]. , 2012, 27(9): 741-744.

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