Drug resistance and virulence characteristics of Salmonella isolated from sterile clinical sites

doi:10.3969/j.issn.1673-8640.2026.08.012

Abstract

Abstract:

Objective To investigate the drug resistance and virulence characteristics of Salmonella isolated from sterile clinical sites，and to provide a reference for clinical diagnosis and treatment. Methods Totally，7 Salmonella isolates were collected from sterile clinical site body fluid samples at Beijing Friendship Hospital of Capital Medical University from January 2023 to May 2024. The isolates were identified by mass spectrometry and automatic bacterial identification and drug susceptibility analyzer. Serological typing was performed by serum agglutination method. Drug resistance genes and virulence genes were determined by polymerase chain reaction（PCR） and sequenced. Results Among the 7 Salmonella isolates，6 isolates were Salmonella enteritidis. The determination rate of multi-drug resistance（MDR） bacteria was 28.57%（2/7）. The Salmonella isolates were sensitive to third-generation cephalosporins and sulfonamides，but resistant or intermediate to quinolones，tetracyclines and penicillins. A total of 4 drug resistance genes were determined，including bla_tem，gyrA，sul2 and tetA，which could mediate resistance to beta-lactamases，quinolones，sulfonamides and tetracyclines. Totally，11 virulence genes were determined，including 7 pathogenicity island genes（sipA，sipC，sopE，invH，sseL，misL and mgtC），1 fimbrial gene（fimA） and 2 virulence plasmid genes（spvB and spvD）. Conclusions The Salmonella isolated from sterile clinical sites have MDR，and the multiple virulence genes carried by them may be related to the invasive infection of Salmonella.

Key words: Salmonella, Salmonella enteritidis, Drug resistance, Virulence gene

CLC Number:

R378.2

CHEN Xinxin, ZHENG Yawen, XU Ying, WEI Jingjuan, LI Yana, CHEN Shuo, LI Hanmei, SUN Wei. Drug resistance and virulence characteristics of Salmonella isolated from sterile clinical sites[J]. Laboratory Medicine, 2026, 41(8): 793-798.

Figures/Tables 5

References 32

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患者编号	性别	年龄/岁	科室	诊断	腹泻（有/无）	粪便常规+潜血	样本来源	菌株鉴定结果	抗菌药物治疗方案
1号	男	49	血管外科	胸主动脉假性动脉瘤	无	黄褐色成型便，潜血阴性	胸腔积液	肠炎沙门菌	美罗培南→ 头孢吡肟
2号	男	74	血管外科	腹主动脉假性动脉瘤	无	黄褐色成型便，潜血阴性	静脉血	肠炎沙门菌	美罗培南
3号	女	89	急诊	菌血症	无	黄褐色成型便潜血阴性	静脉血	肠炎沙门菌	美罗培南→ 头孢唑肟
4号	女	65	急诊病房	菌血症、急性肠炎、重症肌无力	有	水样便，潜血阳性	静脉血	肠炎沙门菌	美罗培南→ 小檗碱
5号	男	71	急诊	菌血症、急性肠炎	有	稀便，潜血阳性	静脉血	曼哈顿沙门菌	美罗培南→ 利福昔明
6号	男	68	心脏中心	腹主动脉假性动脉瘤	无	软黄便，潜血阴性	静脉血	肠炎沙门菌	美罗培南
7号	女	62	重症医学科	胸主动脉假性动脉瘤	无	软黄便，潜血阴性	胸腔积液	肠炎沙门菌	头孢噻肟→ 左氧氟沙星

患者编号	性别	年龄/岁	科室	诊断	腹泻（有/无）	粪便常规+潜血	样本来源	菌株鉴定结果	抗菌药物治疗方案
1号	男	49	血管外科	胸主动脉假性动脉瘤	无	黄褐色成型便，潜血阴性	胸腔积液	肠炎沙门菌	美罗培南→ 头孢吡肟
2号	男	74	血管外科	腹主动脉假性动脉瘤	无	黄褐色成型便，潜血阴性	静脉血	肠炎沙门菌	美罗培南
3号	女	89	急诊	菌血症	无	黄褐色成型便潜血阴性	静脉血	肠炎沙门菌	美罗培南→ 头孢唑肟
4号	女	65	急诊病房	菌血症、急性肠炎、重症肌无力	有	水样便，潜血阳性	静脉血	肠炎沙门菌	美罗培南→ 小檗碱
5号	男	71	急诊	菌血症、急性肠炎	有	稀便，潜血阳性	静脉血	曼哈顿沙门菌	美罗培南→ 利福昔明
6号	男	68	心脏中心	腹主动脉假性动脉瘤	无	软黄便，潜血阴性	静脉血	肠炎沙门菌	美罗培南
7号	女	62	重症医学科	胸主动脉假性动脉瘤	无	软黄便，潜血阴性	胸腔积液	肠炎沙门菌	头孢噻肟→ 左氧氟沙星

菌株编号	头孢他啶	头孢哌酮/ 舒巴坦	头孢吡肟	氨曲南	亚胺培南	美罗培南	环丙沙星	左氧氟沙星	多西环素	米诺环素	复方磺胺甲噁唑	替卡西林/克拉维酸	哌拉西林舒巴坦
1号	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感
2号	敏感	敏感	敏感	敏感	敏感	敏感	敏感	中介	耐药	耐药	敏感	中介	敏感
3号	敏感	敏感	敏感	敏感	敏感	敏感	耐药	中介	耐药	耐药	敏感	耐药	中介
4号	敏感	敏感	敏感	敏感	敏感	敏感	敏感	中介	敏感	敏感	敏感	敏感	敏感
5号	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	中介	敏感	敏感	敏感
6号	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	耐药	敏感
7号	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	中介	敏感

菌株编号	头孢他啶	头孢哌酮/ 舒巴坦	头孢吡肟	氨曲南	亚胺培南	美罗培南	环丙沙星	左氧氟沙星	多西环素	米诺环素	复方磺胺甲噁唑	替卡西林/克拉维酸	哌拉西林舒巴坦
1号	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感
2号	敏感	敏感	敏感	敏感	敏感	敏感	敏感	中介	耐药	耐药	敏感	中介	敏感
3号	敏感	敏感	敏感	敏感	敏感	敏感	耐药	中介	耐药	耐药	敏感	耐药	中介
4号	敏感	敏感	敏感	敏感	敏感	敏感	敏感	中介	敏感	敏感	敏感	敏感	敏感
5号	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	中介	敏感	敏感	敏感
6号	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	耐药	敏感
7号	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	敏感	中介	敏感

抗菌药物类别	耐药表型	耐药或中介菌株数/株	耐药基因型	耐药机制	携带耐药基因菌株数/株	符合率/%
β-内酰胺酶类	TCC	4	bla_tem	产生灭活酶	5	80
喹诺酮类	CIP+LVX	3	gyrA	抗生素作用的靶位改变	5	60
磺胺类	SXT	0	sul2	抗生素作用的靶位改变	6	0
四环素类	DOX、MMO	3	tetA	外排泵	3	100