Correlation between serum Hcy，S100B levels and cerebral metabolite indicators and cognitive impairment in elderly AIS patients

doi:10.3969/j.issn.1673-8640.2025.11.011

Abstract

Abstract:

Objective To investigate the relationship between serum homocysteine（Hcy），S100B protein levels and cerebral metabolite indicators [N-acetylaspartate（NAA）/creatine ratio，choline complex（Cho）/creatine ratio] and cognitive impairment in elderly patients with acute ischemic stroke（AIS）. Methods A total of 92 elderly AIS patients from Shanghai Huangpu Xiangshan Hospital of Traditional Chinese Medicine from January 2021 to June 2023 were enrolled. The clinical data at admission were collected，and hydrogen proton magnetic resonance spectroscopy was used to determine cerebral metabolites [NAA，Cho，creatine]，and the NAA/creatine ratio and Cho/creatine ratio were calculated. Laboratory indicators [Hcy，S100B protein，total cholesterol（TC），triglyceride（TG），low-density lipoprotein cholesterol（LDL-C），high-density lipoprotein cholesterol（HDL-C），interleukin （IL）-1β，IL-6，tumor necrosis factor-alpha（TNF-α），IL-8，IL^-10] were determined. All the patients were followed up for 6 months，and they were classified into cognitive impairment group（46 cases）and non-cognitive impairment group（46 cases）according to the Montreal Cognitive Assessment（MoCA）. Pearson correlation analysis was used to evaluate the correlation between each index and the MoCA score. Results The NAA/creatine ratio in the left and right hemispheres of the brain in cognitive impairment group was lower than that in non-cognitive impairment group（P<0.001），and the Cho/creatine ratio was higher（P<0.01）. The serum Hcy and S100B protein levels in cognitive impairment group were higher than those in non-cognitive impairment group（P<0.05）. There was no statistical significance in serum IL^-1β，IL-6，TNF-α，IL-8，IL^-10，TC，TG，LDL-C and HDL-C levels between the 2 groups（P>0.05）. The NAA/creatine ratio in the left and right hemispheres of the brain in AIS patients was negatively correlated with the MoCA score（r values were -0.13 and -0.20，respectively，P<0.05），and the Cho/creatine ratio was positively correlated with the MoCA score（r values were 0.17 and 0.14，respectively，P<0.05）. Serum Hcy and S100B protein were positively correlated with the MoCA score（r values were 0.47 and 0.21，respectively，P<0.05）. Conclusions The NAA/creatine ratio，Cho/creatine ratio and serum Hcy and S100B protein levels in the brain are related to cognitive impairment in AIS patients and may serve as the biological indicators for assessing the risk of cognitive impairment.

Key words: Homocysteine, S100B protein, N-acetylaspartate/creatine ratio, Choline complex/creatine ratio, Acute ischemic stroke, Cognitive impairment, Elderly

CLC Number:

R446.11

SHEN Zhiying, CHEN Ming, DONG Yueying. Correlation between serum Hcy，S100B levels and cerebral metabolite indicators and cognitive impairment in elderly AIS patients[J]. Laboratory Medicine, 2025, 40(11): 1097-1101.

Figures/Tables 3

References 22

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组别	例数	左侧大脑半球		右侧大脑半球
组别	例数	NAA/肌酸比值	Cho/肌酸比值	NAA/肌酸比值	Cho/肌酸比值
认知障碍组	46	1.03±0.19	1.33±0.23	1.09±0.21	1.31±0.20
无认知障碍组	46	1.28±0.39	1.10±0.12	1.30±0.18	1.16±0.24
t值		3.908	6.013	5.150	3.256
P值		<0.001	<0.001	<0.001	0.002

组别	例数	左侧大脑半球		右侧大脑半球
组别	例数	NAA/肌酸比值	Cho/肌酸比值	NAA/肌酸比值	Cho/肌酸比值
认知障碍组	46	1.03±0.19	1.33±0.23	1.09±0.21	1.31±0.20
无认知障碍组	46	1.28±0.39	1.10±0.12	1.30±0.18	1.16±0.24
t值		3.908	6.013	5.150	3.256
P值		<0.001	<0.001	<0.001	0.002

组别	例数	IL^-1β/（pg·mL^-1）	IL-6/（pg·mL^-1）	TNF-α/（ng·mL^-1）	IL-8/（μg·L^-1）	IL^-10/（μg·L^-1）
认知障碍组	46	63.32±20.44	84.13±20.66	1.31±0.21	10.36±5.16	26.17±10.43
无认知障碍组	46	65.07±18.96	90.27±19.84	1.26±0.33	9.48±7.73	24.55±11.70
t值		0.426	1.454	0.867	0.642	0.701
P值		0.671	0.149	0.388	0.522	0.485

组别	例数	IL^-1β/（pg·mL^-1）	IL-6/（pg·mL^-1）	TNF-α/（ng·mL^-1）	IL-8/（μg·L^-1）	IL^-10/（μg·L^-1）
认知障碍组	46	63.32±20.44	84.13±20.66	1.31±0.21	10.36±5.16	26.17±10.43
无认知障碍组	46	65.07±18.96	90.27±19.84	1.26±0.33	9.48±7.73	24.55±11.70
t值		0.426	1.454	0.867	0.642	0.701
P值		0.671	0.149	0.388	0.522	0.485

组别	例数	TC/ （mmol·L^-1）	TG/ （mmol·L^-1）	LDL-C/ （mmol·L^-1）	HDL-C/ （mmol·L^-1）	Hcy/ （μmol·L^-1）	S100B蛋白/ （μg·L^-1）
认知障碍组	46	4.51±0.78	1.96±1.27	2.95±1.67	1.16±0.49	13.40±3.32	0.36±0.14
无认知障碍组	46	4.79±1.01	1.79±1.12	2.64±1.26	1.25±0.37	9.76±4.30	0.29±0.12
t值		1.488	0.681	1.005	0.994	4.544	2.575
P值		0.140	0.498	0.318	0.323	<0.001	0.012