Abstract:

Objective To analyze the clinical roles of microcyte red blood cell percentage（MicroR%），MicroR%/red blood cell volume distribution width（RDW）-coefficient of variation（CV）ratio combined with other blood routine parameters in the differential diagnosis of iron deficiency anemia（IDA）and thalassemia（TT）. Methods Totally，333 patients with IDA and 40 patients with TT who were initially diagnosed in the Outpatient Department of the Institute of Hematology and Blood Diseases Hospital of Chinese Academy of Medical Sciences from January 2020 to April 2024 were enrolled. A total of 130 healthy subjects from Tianjin Neoclass Specialized Health Assessment Center were enrolled as healthy control group. The differences in blood routine test results among the groups were compared. Pearson correlation analysis was used to evaluate the correlation of each index. Receiver operating characteristic（ROC）curve was used to evaluate the efficacy of each index in the diagnosis of IDA and TT. Results There was statistical significance in hematocrit （HCT），hemoglobin （Hb），RDW-CV，red cell distribution width-standard deviation（RDW-SD），mean corpuscular volume（MCV），mean corpuscular hemoglobin（MCH），mean corpuscular hemoglobin concentration（MCHC）and MicroR% between anemia group and healthy control group（P<0.01）. There was statistical significance in RBC，HCT，Hb，MCV，MCH，MCHC，MicroR%/RDW-CV ratio between IDA patients and TT patients（P<0.01）. MCV was negatively correlated with MicroR%（r=-0.945，P<0.001），and it was positively correlated with MicroR%/RDW-CV ratio（r=0.208，P<0.001）. The areas under curves of MicroR% and MicroR%/RDW-CV ratio in the diagnosis of IDA and TT were 0.988，0.673 and 0.981，1.000，respectively. Conclusions MicroR% and MicroR%/RDW-CV ratio have good clinical value in the preliminary screening of small cell hypochromic anemia. The combined application of blood routine parameters can initially diagnose IDA and TT.

Key words: Blood routine parameter, Iron deficiency anemia, Thalassemia