Abstract:

Objective To establish homogeneous method for determining remnant lipoprotein-cholesterol（RLP-C）,and to evaluate it primarily. Methods A homogeneous method for the determination of RLP-C was established based on the screening of non-lipoproteins with polyoxyethylene-polyethylene oxide butene（POE-POB） as screening agent. The self-established method was compared with the reference method [RLP-C reagent（homogeneous method） of Japan Concorde company]. Serum RLP-C and triglyceride（TG）,total cholesterol（TC）,apolipoprotein C2（apo C2） were determined in 116 patients with mixed hyperlipidemia and 130 healthy controls. Pearson correlation analysis was used to evaluate their correlations. Results The optimal buffer system was 3-morpholinopropanesulfonic acid（MOPS） buffer system. The optimal pH value was 6.8,the optimal concentration of POE-POB was 8 g/L,and the determination delay time was 5 min. Intra-self established method coefficient of value（CV） was 3.37%,and inter-CV was 4.91%. The reagent was stable for 1 month at 4 ℃. The results of reference method and self-established method were slightly different,but the correlation between the 2 methods was good（r=0.980）. Bland-Altman scatter plot showed that 4（4.08%） samples were not within ±1.96s. The concentrations of RLP-C,TG,apo C2 and TC in mixed hyperlipidemia group were higher than those in healthy control group（P<0.001）. RLP-C was positively correlated with TG（r=0.496,P<0.001）,but it was not correlated with apo C2 and TC（r=0.157 and -0.090,P>0.05）. Conclusions The self-established method is simple and rapid,which can be used in automatic biochemical analyzer and be used as clinical routine test.

Key words: Remnant lipoprotein-cholesterol, Homogeneous method, Polyoxyethylene-polyethylene oxide butene, Hyperlipidemia