Application of resazurin reduction-based assay for the rapid detection of polymyxin B drug susceptibility in vitro in Acinetobacter baumannii and Pseudomonas aeruginosa

doi:10.3969/j.issn.1673-8640.2019.09.010

Laboratory Medicine ›› 2019, Vol. 34 ›› Issue (9): 812-814.DOI: 10.3969/j.issn.1673-8640.2019.09.010

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Application of resazurin reduction-based assay for the rapid detection of polymyxin B drug susceptibility in vitro in Acinetobacter baumannii and Pseudomonas aeruginosa

WU Xinglong¹, ZHAO Xiangwen¹, ZHAO Qingling²

1. Department of Clinical Laboratory,the People's Hospital of Lanling County,Lanling 277799,Shandong,China
2. Department of Clinical Laboratory,Shandong Rongjun General Hospital,Jinan 250013,Shandong,China

Received:2019-01-02 Online:2019-09-30 Published:2019-09-29

Abstract

Abstract:

Objective To evaluate the feasibility of resazurin reduction-based assay for the rapid detection of polymyxin B drug susceptibility in vitro in Acinetobacter baumannii and Pseudomonas aeruginosa. Methods A total of 112 isolates of Acinetobacter baumannii and Pseudomonas aeruginosa were collected,and the polymyxin B drug susceptibility in vitro was performed by resazurin reduction-based assay and standard broth microdilution method. The performance of resazurin reduction-based assay was evaluated. Results Using broth microdilution method as standard,the accuracy and the positive detection rate of resazurin reduction-based assay were 98.2%（110/112） and 100%（33/33）,respectively. All drug resistance results can be obtained within 60 min after adding resazurin. Conclusions Polymyxin B drug susceptibility in vitro in Acinetobacter baumannii and Pseudomonas aeruginosa is rapid,highly sensitive,specific,easy-to-perform and inexpensive,which could be applied in clinic widely.

Key words: Resazurin, Drug susceptibility test in vitro, Rapid detection, Polymyxin B

CLC Number:

R446.1

WU Xinglong, ZHAO Xiangwen, ZHAO Qingling. Application of resazurin reduction-based assay for the rapid detection of polymyxin B drug susceptibility in vitro in Acinetobacter baumannii and Pseudomonas aeruginosa[J]. Laboratory Medicine, 2019, 34(9): 812-814.

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Application of resazurin reduction-based assay for the rapid detection of polymyxin B drug susceptibility in vitro in Acinetobacter baumannii and Pseudomonas aeruginosa

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