Application of SYSMEX XE-5000 automatic blood analyzer for the routine determination of cerebrospinal fluid among children

doi:10.3969/j.issn.1673-8640.2016.09.016

Abstract

Abstract:

Objective To investigate the significance of SYSMEX XE-5000 automatic blood analyzer for the routine determination of cerebrospinal fluid among children. Methods A total of 194 cerebrospinal fluid samples were collected and determined by XE-5000 and manual microscopy. The consistency was evaluated. Results When white blood cell（WBC） count was ≥30 cells/μL,there was no statistical significance between the 2 methods（P>0.05）. However,when WBC count was <30 cells/μL,there was a statistical significance between the 2 methods（P<0.05）. When WBC count was ≥10 cells/μL,there were good correlations in counting mononuclear cells and multinuclear cells between the 2 methods （P<0.01）. When red blood cell（RBC） count was >1 000 cells/μL,there was a good correlation between the 2 methods（r=0.98,P<0.01）. Conclusions XE-5000 can be used in the determination of cerebrospinal fluid among children,when WBC count is ≥30 cells/μL.

Key words: Cell counting, Cerebrospinal fluid, Children, SYSMEX XE-5000 automatic blood analyzer

CLC Number:

R446.1

SUN Kuixia, YANG Wenshuang, JIANG Shiju. Application of SYSMEX XE-5000 automatic blood analyzer for the routine determination of cerebrospinal fluid among children[J]. Laboratory Medicine, 2016, 31(9): 807-809.

Figures/Tables 2

References 8

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WBC计数（个/μL）	例数	手工法	XE-5000	P值
<30	160	2.00（0.00~6.00）	4.00（1.00~10.00）	<0.05
≥30	34	99.00（47.50~280.00）	102.00（50.30~284.00）	>0.05

WBC计数（个/μL）	例数	手工法	XE-5000	P值
<30	160	2.00（0.00~6.00）	4.00（1.00~10.00）	<0.05
≥30	34	99.00（47.50~280.00）	102.00（50.30~284.00）	>0.05

WBC计数（个/μL）	例数	单个核细胞				多个核细胞
WBC计数（个/μL）	例数	手工法	仪器法	r值	P值	手工法	仪器法	r值	P值
<10^*	128	0.00（0.00~2.00）	1.00（1.00~4.00）	0.48	<0.01	0.00（0.00~1.00）	1.00（0.00~1.00）	0.32	<0.01
10~100^*	51	14.00（8.50~21.50）	18.00（10.00~27.50）	0.93	<0.01	20.00（14.50~30.00）	26.00 （17.50~38.50）	0.79	<0.01
≥100^#	15	56.00（32.00~78.00）	54.00 （35.00~77.00）	0.93	<0.01	40.00 （20.00~68.00）	42.00 （22.00~65.00）	0.94	<0.01

WBC计数（个/μL）	例数	单个核细胞				多个核细胞
WBC计数（个/μL）	例数	手工法	仪器法	r值	P值	手工法	仪器法	r值	P值
<10^*	128	0.00（0.00~2.00）	1.00（1.00~4.00）	0.48	<0.01	0.00（0.00~1.00）	1.00（0.00~1.00）	0.32	<0.01
10~100^*	51	14.00（8.50~21.50）	18.00（10.00~27.50）	0.93	<0.01	20.00（14.50~30.00）	26.00 （17.50~38.50）	0.79	<0.01
≥100^#	15	56.00（32.00~78.00）	54.00 （35.00~77.00）	0.93	<0.01	40.00 （20.00~68.00）	42.00 （22.00~65.00）	0.94	<0.01

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