Laboratory Medicine ›› 2015, Vol. 30 ›› Issue (2): 201-203.DOI: 10.3969/j.issn.1673-8640.2015.02.023
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Received:2014-12-25
Online:2015-02-28
Published:2015-02-12
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URL: https://www.shjyyx.com/EN/10.3969/j.issn.1673-8640.2015.02.023
| 方法 | 第一次实验 | 第二次实验 | 第三次实验 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATCC 43596 | VPI 10463 | ATCC 43596 | VPI 10463 | ATCC 43596 | VPI 10463 | |||||||||
| 96 h | 120 h | 96 h | 120 h | 96 h | 120 h | 96 h | 120 h | 96 h | 120 h | 96 h | 120 h | |||
| 平板涂布法 | 30.3 | 45.3 | 20.5 | 41.5 | 28.5 | 48.5 | 13.7 | 36.7 | 17.2 | 35.2 | 12.5 | 28.1 | ||
| 相差显微镜法 | 15.6 | 35.3 | 8.7 | 28.5 | 12.1 | 37.2 | 10.2 | 26.2 | 12.3 | 32.5 | 9.3 | 28.7 | ||
| 孔雀绿染色法 | 14.6 | 32.6 | 10.1 | 30.2 | 13.7 | 35.3 | 10.5 | 26.3 | 11.8 | 30.3 | 8.8 | 28.2 |
| 方法 | 第一次实验 | 第二次实验 | 第三次实验 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATCC 43596 | VPI 10463 | ATCC 43596 | VPI 10463 | ATCC 43596 | VPI 10463 | |||||||||
| 96 h | 120 h | 96 h | 120 h | 96 h | 120 h | 96 h | 120 h | 96 h | 120 h | 96 h | 120 h | |||
| 平板涂布法 | 30.3 | 45.3 | 20.5 | 41.5 | 28.5 | 48.5 | 13.7 | 36.7 | 17.2 | 35.2 | 12.5 | 28.1 | ||
| 相差显微镜法 | 15.6 | 35.3 | 8.7 | 28.5 | 12.1 | 37.2 | 10.2 | 26.2 | 12.3 | 32.5 | 9.3 | 28.7 | ||
| 孔雀绿染色法 | 14.6 | 32.6 | 10.1 | 30.2 | 13.7 | 35.3 | 10.5 | 26.3 | 11.8 | 30.3 | 8.8 | 28.2 |
| [1] | FREEMAN J, BAUER MP, BAINES SD, et al.The changing epidemiology of Clostridium difficile infections[J]. Clin Microbiol Rev,2010,23:529-549. |
| [2] | 孙静,陈建华.枯草芽孢杆菌形成芽孢时母细胞中基因表达的调控[J].生物技术,2007,17:79-83. |
| [3] | MERRIGAN M, VENUGOPA A, MALLOZZI M, et al.Human Hypervirulent Clostridium difficile Strains Exhibit Increased Sporulation as Well as Robust Toxin Production[J]. J Bacteriol, 2010,192:4904-4911. |
| [4] | VOHRA P, POXTON I.Comparison of toxin and spore production in clinically relevant strains of Clostridium difficile[J]. Microbiology, 2011,157:1343-1353. |
| [5] | BURNS DA, HEAP JT, MINTON NP.The diverse sporulation characteristics of Clostridium difficile clinical isolates are not associated with type[J]. Anaerobe,2010,16:618-622. |
| [6] | SORG JA, SONENSHEIN AL.Inhibiting the initiation of Clostridium difficile spore germination using analogs of chenodeoxycholic acid, a bile acid[J]. J Bacteriol, 2010,192:4983-4990. |
| [7] | 宋淑贤,聂清.微生物学常用细菌染色方法的改进[J].黑龙江医药科学,2001,24:36. |
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