Esablishment on isolation and identification kit for circulating tumor cells in breast cancer

doi:10.3969/j.issn.1673-8640.2015.10.012

Abstract

Abstract: Objective

To investigate the clinical application significance of epithelial cell adhesion molecule (EpCAM)lipid immune magnetic particles and human breast mammaglobin (hMAM) monoclonal antibody in breast cancer circulating tumor cell (CTC) isolation and identification kit.

Methods

Breast cancer MCF-7 cells were mixed in healthy fresh blood samples, and the isolation ability of EpCAM lipid immune magnetic particles on breast cancer was analyzed by immune magnetic particle isolating technique. A comparison between EpCAM lipid immune magnetic particles and Cell Tracks^® AutoPrep^® System (CellSearch^TM) magnetic particles for the capture efficiency of MCF-7 cells was performed in a simulation experiment, and the hematological items were determined.

Results

The results of fluorescence microscopy showed that the capture ability of EpCAM lipid immune magnetic particle was significantly stronger than that of nanoparticles without antibody. The results of laser scanning confocal microscopy showed that the modification of EpCAM lipid immune magnetic particle antibody significantly enhanced the efficiency and specificity of magnetic particles capturing MCF-7 cells. Simulation experiment results showed that the capturing results of EpCAM lipid immune magnetic particles of 10, 20, 40, 80, 150 and 200 MCF-7 cells were close to CellSearch^TM magnetic particles. In the blood samples from patients with breast cancer, the CTC capturing efficiencies of EpCAM lipid immune magnetic particles and CellSearch^TM magnetic particles were almost the same, in some cases, the CTC capturing efficiency of EpCAM lipid immune magnetic particles was significantly better than that of CellSearch^TM magnetic particles.

Conclusions

The research on the establishment of EpCAM lipid immune magnetic particles and hMAN monoclonal antibody based on CTC isolation and identification kit has high capturing efficiency of CTC in blood samples from patients with breast cancer. It will provide a strong support for the early diagnosis, pre-operation and post-operation analysis and treatment effect evaluation.

Key words: Circulating tumor cell, Epithelial cell adhesion molecule, Immune magnetic particle, Human breast mammaglobin

CLC Number:

R446.1

ZHANG Jian, FENG Xiaoyan, SUN Tingting, WANG Kai, ZHANG Xirui, GAO Jiefeng, LIANG Xiaofei, KANG Xiangdong, PENG Junjie, ZHANG Heqiu, MIAO Chaoliang, SHEN Hebai. Esablishment on isolation and identification kit for circulating tumor cells in breast cancer[J]. Laboratory Medicine, 2015, 30(10): 1011-1016.

Figures/Tables 8

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