Genotype of methicillin-resistant Staphylococcus aureus from Mongolian in Ordos

doi:10.3969/j.issn.1673-8640.2014.02.005

Abstract

Abstract:

Objective To investigate the molecular prevalence characteristics of methicillin-resistant Staphylococcus aureus (MRSA) from Mongolian in Ordos, and to investigate the genotypes and distribution pattern of MRSA isolates. Methods A total of 54 MRSA isolates were collected from January 2009 to August 2011.The multiplex polymerase chain reaction(PCR) was used to determine Staphylococcal cassette chromosome mec (SCCmec) genotypes, Panton-Valentine leukocidin (PVL) gene(pvl) and multi-locus sequence typing (MLST) of MRSA isolates. Results The main SCCmec Ⅰ-Ⅴ genotypes of MRSA isolates, accounted for 0.00%, 50.00%, 46.30%, 1.85% and 1.85%, respectively. The pvl gene had been discovered in only 1 isolate. Among 24 MRSA isolates, MLST results showed that 13 isolates were ST239(54.17%), 9 isolates were ST5(37.50%), 1 isolate was ST59 (4.17%), and 1 isolate was ST7 (4.17%). Conclusions The main genotypes of MRSA are SCCmec Ⅱ and SCCmec Ⅲ from Mongolian in Ordos.

Key words: Methicillin-resistant Staphylococcus aureus, Staphylococcal cassette chromosome mec, Multi-locus sequence typing, Panton-Valentine leukocidin

CLC Number:

R378.1

WANG Yanhai, DUAN Baosheng, BA Teer, ZHANG Jing, ZHAO Na.. Genotype of methicillin-resistant Staphylococcus aureus from Mongolian in Ordos[J]. , 2014, 29(2): 110-114.

References

[1] Forcade NA, Parchman ML, Jorgensen JH, et al. Prevalence, severity, and treatment of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) skin and soft tissue infections in 10 medical clinics in Texas: a South Texas Ambulatory Research Network (STARNet) study[J]. J Am Board Fam Med, 2011, 24(5):543-550.
[2] Nonga BN, Jemea B, Tambo FM, et al. Feasibility of a simple drainage system in Cameroonian children after thoracotomy and decortication for empyema thoracis[J]. Afr J Paediatr Surg, 2012, 9(1):27-31.
[3] Chongtrakool P, Ito T, Ma XX, et al. Staphylococcal cassette chromosome mec(SCCmec) typing of methicillin-resistant Staphylococcus aureus strains isolated in 11 Asian countries: a proposal for a new nomenclature for SCCmec elements[J]. Antimicrob Agents Chemother, 2006, 50(3):1001-1012.
[4] Clinical and Laboratory Standards Institute. Perfor-mance standards for antimicrobial testing; twenty-second informational supplement[S]. M100-S22, CLSI, 2008.
[5] Zhang K, McClure JA, Elsayed S, et al. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types Ⅰ to Ⅴ in methicillin-resistant Staphylococcus aureus[J]. J Clin Microbiol, 2005, 43(10):5026-5033.
[6] Lina G, Piémont Y, Godail-Gamot F, et al. Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia[J]. Clin Infect Dis, 1999, 29(5):1128-1132.
[7] Enright MC, Day NP, Davies CE, et al. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphy-lococcus aureus[J]. J Clin Microbiol, 2000, 38(3): 1008-1015.
[8] Vyas K, Hospenthal DR, Mende K, et al. Recurrent community-acquired methicillin-resistant Staphy-lococcus aureus infections in an HIV-infected person[J]. J Clin Microbiol, 2011, 49(5):2047-2053.
[9] Li S, Skov RL, Han X, et al. Novel types of staphylococcal cassette chromosome mec elements identified in clonal complex 398 methicillin-resistant Staphy-lococcus aureus strains[J]. Antimicrob Agents Chemother, 2011, 55(6):3046-3050.
[10] 师志云, 赵志军, 贾伟, 等. 耐甲氧西林金黄色葡萄球菌分子流行病学研究[J]. 中华医院感染学杂志, 2011, 21(14):2886-2888.
[11] Ko KS, Lee JY, Suh JY, et al. Distribution of major genotypes among methicillin-resistant Staphylococcus aureus clones in Asian countries[J]. J Clin Micro-biol, 2005, 43(1):421-426.
[12] 吴爱武, 招志翔, 林红燕. 金黄色葡萄球菌的药敏表型和耐甲氧西林金黄色葡萄球菌的SCCmec基因分型[J]. 检验医学, 2010, 25(12):934-939.
[13] Huijsdens XW, van Santen-Verheuvel MG, Spalburg E, et al. Multiple cases of familial transmission of community-acquired methicillin-resistant Staphy-lococcus aureus[J]. J Clin Microbiol, 2006, 44(8):2994-2996.
[14] Hou Z, Zhou Y, Wang H, et al. Co-blockade of mecR1/blaR1 signal pathway to restore antibiotic susceptibility in clinical isolates of methicillin-resistant Staphylococcus aureus[J]. Arch Med Sci, 2011, 7(3): 414-422.
[15] Nass T, Fortineau N, Spicq C, et al. Three-year survey of community-acquired methicillin-resistant Staphylococcus aureus producing Panton-Valentine leukocidin in a French university hospital[J]. J Hosp Infect, 2005, 61(4):321-329.
[16] Boyle-Vavra S, Daum RS. Community-acquired methicillin-resistant Staphylocococcus aureus: the role of Panton-Valentine leukocidin[J]. Lab Invest, 2007, 87(1):3-9.
[17] 林萍, 孙阳, 韩立中, 等. 杀白细胞素pvl基因阴性MRSA对抗菌药物的耐药性研究[J]. 检验医学, 2011, 26(5):295-298.
[18] Cha HY, Moon DC, Choi CH, et al. Prevalence of the ST239 clone of methicillin-rethicllin-resistant Staphylococcus aureus and differences in antimicrobial susceptibilities of ST239 and ST5 clones identified in a Korean hospital[J]. J Clin Microbiol, 2005, 43(8):3610-3614.
[19] McClure JA, Conly JM, Lau V, et al. Novel muhiplex PCR assay for detection of the staphylococcal virulence marker Panton-Valentine leukocidin genes and simultaneous discrimination of methicillin-susceptible from-resistant staphylococci[J]. J Clin Microbiol, 2006, 44(3):1141-1144.