›› 2012, Vol. 27 ›› Issue (9): 749-753.

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Research on six genes and insertion sequence ISAbal of carbopenems-resistant Acinetobacter baumannii

  

  1. 1. Department of Clinical Laboratory,the First Affiliated Hospital of Nanchang University,Jiangxi Nanchang 330006,China; 2. Department of Respiration,the First Affiliated Hospital of Nanchang University,Jiangxi Nanchang 330006,China
  • Received:2012-07-03 Revised:2012-08-31 Online:2012-09-12 Published:2012-09-12

Abstract: Objective To study the relationship between the genotype characteristics and insertion sequence ISAbal of carbopenems-resistant Acinetobacter baumannii(CRAB),and investigate the drug-resistant mechanism.  Methods A total of 70 strains of CRAB (no repeat strains) and 10 strains of carbopenems-sensitive Acinetobacter baumannii were collected. Metalloenzyme beta-lactamase (MBLs) were detected by 2-mercaptopropionic acid inhibition test. The blaIMP,blaVIM,blaOXA-23,blaOXA-24,blaOXA-51,blaOXA-58 and ISAbalOXA-23 were amplified by multiplex polymerase chain reaction (PCR). The amplified products were measured for DNA sequence.  Results The synergy tests were negative (MBLs negative) by 2-mercaptopropionic acid and ceftazime in 70 strains of CRAB. Through 7 pairs of specific primers,70 strains of CRAB and 10 strains of carbopenems-sensitive Acinetobacter baumannii were determined for PCR amplification. All the CRAB strains carried blaOXA-23 and blaOXA-51,but blaOXA-24 was never detected. Only 1 strain carried blaOXA-58,and the 80 strains did not carry blaIMP and blaVIM. In addition,all the CRAB strains carried insertion sequence ISAbalOXA-23,however,all the sensitive strains did not carry.  Conclusions Major genes are blaOXA-51 and blaOXA-23 in the CRAB strains. Insertion sequence ISAbal is located in the upstream of blaOXA-23. There is no correlation among drug-resistance of Acinetobacter baumannii to MBLs,blaOXA-24 and blaOXA-58.

Key words: mso-bidi-font-size: 11.0pt, mso-ascii-theme-font: minor-latin, mso-fareast-theme-font: minor-fareast, mso-hansi-theme-font: minor-latin, mso-bidi-font-family: 'Times New Roman', mso-bidi-theme-font: minor-bidi, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体" lang="EN-US">Acinetobacter baumanniifont-size: 7.5pt, mso-fareast-theme-font: minor-fareast, mso-bidi-font-family: 'Times New Roman', mso-bidi-theme-font: minor-bidi, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体, mso-font-kerning: 0pt" lang="EN-US">, mso-bidi-font-size: 11.0pt, mso-ascii-theme-font: minor-latin, mso-fareast-theme-font: minor-fareast, mso-hansi-theme-font: minor-latin, mso-bidi-font-family: 'Times New Roman', mso-bidi-theme-font: minor-bidi, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体" lang="EN-US">Metalloenzymefont-size: 7.5pt, mso-fareast-theme-font: minor-fareast, mso-bidi-font-family: 'Times New Roman', mso-bidi-theme-font: minor-bidi, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体, mso-font-kerning: 0pt" lang="EN-US">, mso-bidi-font-size: 11.0pt, mso-ascii-theme-font: minor-latin, mso-fareast-theme-font: minor-fareast, mso-hansi-theme-font: minor-latin, mso-bidi-font-family: 'Times New Roman', mso-bidi-theme-font: minor-bidi, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体" lang="EN-US">Carbopenemfont-size: 7.5pt, mso-fareast-theme-font: minor-fareast, mso-bidi-font-family: 'Times New Roman', mso-bidi-theme-font: minor-bidi, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体, mso-font-kerning: 0pt" lang="EN-US">, mso-bidi-font-size: 11.0pt, mso-ascii-theme-font: minor-latin, mso-fareast-theme-font: minor-fareast, mso-hansi-theme-font: minor-latin, mso-bidi-font-family: 'Times New Roman', mso-bidi-theme-font: minor-bidi, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体" lang="EN-US"> Genotypefont-size: 7.5pt, mso-fareast-theme-font: minor-fareast, mso-bidi-font-family: 'Times New Roman', mso-bidi-theme-font: minor-bidi, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体, mso-font-kerning: 0pt" lang="EN-US">, mso-bidi-font-size: 11.0pt, mso-ascii-theme-font: minor-latin, mso-fareast-theme-font: minor-fareast, mso-hansi-theme-font: minor-latin, mso-bidi-font-family: 'Times New Roman', mso-bidi-theme-font: minor-bidi, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体" lang="EN-US">Insertion sequence ISAbal