Abstract: Objective　To study the influence of different bacteria or fungi with different levels on the determination of urinary red blood cell（RBC） count by UF-1000i urinary sediment analyzer（UF-1000i），so as to provide more reliable laboratory evidence in the diagnosis and follow-up of urinary tract diseases.  Methods　The urine RBC counts were analyzed by UF-1000i from urine mixture specimens and hematuria specimens with Blastomyces albicans，in parallel with manual microscopy respectively.  Results　The Gram-negative bacilli（Escherichia coli，Klebsiella pneumonia and Pseudomonas aeruginosa） and Gram-positive cocci （Staphylococcus aureus） isolated from urine specimens of urinary tract infection and low-concentration Blastomyces albicans（<106 mL） were not impact the urine RBC count detected by UF-1000i. High concentration bacteriuria specimens（=109/mL） of Enterococcus faecalis caused the false-positive RBC count. The urine RBC count determined by UF-1000i was caused false-positive by Candida albicans, when the concentration of fungi was more than 106/mL. The concentration of Candida albicans reached to 108/mL，and the hematuria specimen RBC count would falsely increased（P=0.006）.  Conclusions　UF-1000i has special bacterial examination channel, and it could prevent the interference with urine RBC count obviously. However, it is hard to prevent the interference of high concentration fungi，which would cause false-positive urine RBC count. It is recommended to correct the RBC count by manual microscopy in order to provide accurate laboratory results.