Abstract: Objective　To investigate the immunophenotype characteristics of acute leukemia(AL) . Methods　CD45/ side scatter (SSC) gating strategy and multiparametric flow cytometry were used to determine immunophenotype of 89 patients with AL. Results　In acute myeloid leukemia (AML)， the cluster of antigen total percentages and positive cell percentages in patients with positive expression were CD13（96.7%,68.6%±22.7%）,CD33（95.0%,72.6%±23.5%）,myeloperoxidase (cMPO)（90.0%,57.5%±26.9%） and CD117（73.3%,33.7%±14.4%）. In acute B lymphoblastic leukemia (B-ALL)， the cluster of antigen total percentages and positive cell percentages in patients with positive expression were cCD79a（96.2%,69.8%±22.1%）,CD19(96.2%,67.4%±23.2%) and CD10(53.8%,66.7%±15.4%). In acute T lymphoblastic leukemia (T-ALL)，the cluster of antigen total percentages and positive cell percentages in patients with positive expression were cCD3(100.0%,53.6%±18.9%) and CD7(66.7%,92.0%±0.5%). Conclusions　There are differences of the cluster of antigen total percentages and positive cell percentages in patients with positive expression in all subtypes of AL， which can be used for the reference of the immunophenotype diagnosis by flow cytometry.

Key words: Immunophenotype, Flow cytometry, Acute leukemia