The polymorphism determination of T1128C in neuropeptide Y gene among Han population in Shanghai

Abstract

Abstract: Objective To investigate the distribution frequency of T1128C (Leu7Pro) single-nucleotide polymorphism (SNP) in neuropeptide Y (NPY) gene among Han population in Shanghai. Methods Polymerase chain reaction (PCR)-ligase detection reaction (LDR) was performed to detect the T1128C polymorphism in NPY gene among 867 Han people in Shanghai. A pair of primers and 3 probes were designed according to target DNA sequence. The LDR was performed after PCR amplification, and the genotypes were analyzed according to the length of fragments of LDR products on a sequencer by electrophoresis. Results All 867 subjects showed T1128T(Leu7Leu) but no T1128C(Leu7Pro) or C1128C(Pro7Pro) in the NPY gene.〖WTHZ〗 Conclusions〖WTBZ〗 There is difference of T1128C polymorphism in NPY gene among populations with different genetic and geographic backgrounds. There is no T1125C (Leu7Pro) polymorphism in NPY gene among Shanghai Han population. There is no correlation of T1128C polymorphism in NPY gene with coronary heart disease among Shanghai Han population.

Key words: Neuropeptide Y gene, Single-nucleotide polymorphism, Polymerase chain reaction, Ligase detection reaction, Neuropeptide Y gene, Single-nucleotide polymorphism, Polymerase chain reaction, Ligase detection reaction

MIAO Yingxin;GAN Jiemin;SHI Hong;CHEN Jie;ZHOU Xiaoqian;ZHAO Hu. The polymorphism determination of T1128C in neuropeptide Y gene among Han population in Shanghai[J]. , 2012, 27(4): 304-307.

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