Abstract: Objective  To investigate the influence of adiponectin（APN） on aldehyde oxidase-1 （AOX1） expression of HepG2 hepatoma cell lines and HL-7701 hepatic cell lines incubated by saturated fatty acid (SFA), and discuss the curative effect and possible mechanism of APN to protect non-alcohol fatty liver disease（NAFLD）.  Methods  Fatty HepG2 hepatoma cell lines and HL-7701 hepatic cell lines were incubated by 100 μmol/L SFA, and cells without being incubated by 100 μmol/L SFA were as controls. The fatty HepG2 hepatoma cell lines and HL-7701 hepatic cell lines were performed the treatment of APN (0.5,1.0,1.5 and 2.5 μg/mL), and cells without APN treatment were used as control group. Reverse transcription-polymerase chain reaction（RT-PCR） and Western-blot were utilized to detect the expressions of AOX1 gene and AOX1 protein.  Results  The AOX1 gene and AOX1 protein expressions of cells incubated by 100 μmol/L SFA were significantly higher than those of controls without being incubated by 100 μmol/L SFA (P<0.05), and the expression of HepG2 cells was significantly higher than that of HL-7701 cells (P<0.05). The AOX1 gene and AOX1 protein expressions of cells treated by APN were reduced significantly comparing to those without APN treatment (P<0.05)， which indicated that APN could decrease the expression effectively. However， the AOX1 expression of cells had no change, when the APN concentration reached 1.5 μg/mL. The AOX1 gene and AOX1 protein expressions of HepG2 cells decreased significantly higher than those of HL-7701 cells at the same APN concentration. Conclusions  APN could reduce the expression of cells incubated by saturated fatty acid effectively， therefore it could protect hepatic cells from NAFLD.

Key words: Adiponectin, Aldehyde oxidase-1, HepG2 hepatoma cell line, HL-7701 hepatic cell line, Non-alcohol fatty liver disease