Abstract:

Objective To investigate the predictive roles of serum epithelial cell adhesion molecule（EpCAM），soluble epithelial cadherin（sE-Cad）and FAM64A mRNA for recurrence and metastasis in ovarian cancer patients after laparoscopic radical surgery. Methods A total of 100 patients with ovarian cancer who underwent laparoscopic radical surgery at Qinghai Red Cross Hospital from January 2022 to January 2023 were enrolled. The clinical data were collected，and the levels of serum EpCAM and sE-Cad before surgery and the relative expression level of FAM64A mRNA were determined. All the patients were followed up for 1 year，and they were classified into recurrence and metastasis group（14 cases）and non-recurrence and metastasis group（86 cases）. Receiver operating characteristic（ROC）curve was used to evaluate the efficacy of serum EpCAM，sE-Cad combined with FAM64A mRNA in predicting recurrence and metastasis in ovarian cancer patients after surgery. Results There was a statistical significance in the International Federation of Gynecology and Obstetrics（FIGO）stages between recurrence and metastasis group and non-recurrence and metastasis group（P<0.05），while there was no statistical significance in the other clinical data（P>0.05）. The levels of serum EpCAM，sE-Cad and FAM64A mRNA relative expression in stage Ⅲ ovarian cancer patients were higher than those in stage Ⅱ ovarian cancer patients（P<0.05）. The levels of serum EpCAM，sE-Cad and FAM64A mRNA relative expression in recurrence and metastasis group were higher than those in non-recurrence and metastasis group（P<0.05）. The areas under curves（AUC）of single and combined determinations of serum EpCAM，sE-Cad and FAM64A mRNA for predicting recurrence and metastasis in ovarian cancer patients after surgery were 0.802，0.793，0.736 and 0.947，respectively. Conclusions The levels of serum EpCAM，sE-Cad and FAM64A mRNA expression are increased in ovarian cancer patients with recurrence and metastasis after laparoscopic radical surgery. The combined determination has a high predictive value for recurrence and metastasis in ovarian cancer patients after surgery.

Key words: Epithelial cell adhesion molecule, Soluble epithelial cadherin, FAM64A gene, Laparoscopic ovarian cancer radical surgery, Recurrence and metastasis