Application of multiple methods for Eperythrozoon determination

doi:10.3969/j.issn.1673-8640.2019.04.014

Abstract

Abstract:

Objective To improve the determination specificity of human Eperythrozoon through multiple methods. Methods A total of 223 patients with blood diseases were enrolled. Eperythrozoon was determined by Wright-Giemsa staining and acridine staining. The determination rates were compared between the 2 methods. The cases with both positive results were determined by polymerase chain reaction（PCR）. The positive results of PCR were analyzed by sequencing and GenBank comparison. Results The positive rates were 46.19% and 28.25% by Wright-Giemsa staining and acridine staining,respectively. In 40 cases with both positive results,10 cases were positive by PCR,and 1 case with dark stripe was sequenced. The homology of 16S rRNA sequences between this case and Mycoplasma suis was 95.84%,that for Mycoplasma bovis was 86.84%,and that for Mycoplasma capricolum was 87.33%. Conclusions The multiple methods for Eperythrozooncould improve determination specificity.

Key words: Eperythrozoon, Wright-Giemsa staining, Acridine staining, Polymerase chain reaction

CLC Number:

R446.1

LU Lingyi, WANG Jianbiao, YAO Yufeng. Application of multiple methods for Eperythrozoon determination[J]. Laboratory Medicine, 2019, 34(4): 351-354.

Figures/Tables 5

References 11

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吖啶橙荧光染色	瑞氏-吉姆萨染色		合计
吖啶橙荧光染色	阳性	阴性	合计
阳性	40（17.94）	23（10.31）	63（28.25）
阴性	63（28.25）	97（43.50）	160（71.75）
合计	103（46.19）	120（53.81）	223（100.00）

吖啶橙荧光染色	瑞氏-吉姆萨染色		合计
吖啶橙荧光染色	阳性	阴性	合计
阳性	40（17.94）	23（10.31）	63（28.25）
阴性	63（28.25）	97（43.50）	160（71.75）
合计	103（46.19）	120（53.81）	223（100.00）

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