Pyro-sequencing analysis for the detection of BRAF gene mutation

doi:10.3969/j.issn.1673-8640.2018.12.016

Abstract

Abstract:

Objective To establish a pyro-sequencing analysis（PYRO） for the detection of V-raf murine sarcoma viral oncogene homolog B1（BRAF） gene mutation.Methods The gDNA was extracred from 100 colorectal cancer patients'tissue samples. PYRO BRAF for gene was performed through Qigene pyro24. The accuracy was validated by repeat test and Sanger sequencing.Results The PYRO for the detection of BRAF gene mutation was established. Repeat test and Sanger sequencing showed that the accuracy and reliability were good. The detected gene mutation in 100 colorectal cancer patients' tissue samples accounted for 6%.Conclusions The established PYRO for the detection of BRAF gene mutation is rapid,simple,sensitive and accurate.

Key words: Pyro-sequencing, V-raf murine sarcoma viral oncogene homolog B1, Colorectal cancer, Gene mutation

CLC Number:

R446.1

ZHOU Gang, ZHONG Yan. Pyro-sequencing analysis for the detection of BRAF gene mutation[J]. Laboratory Medicine, 2018, 33(12): 1127-1131.

Figures/Tables 4

References 14

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引物	引物名称	引物序列（5'~3'）
PCR扩增引物	BrafPyro F	TCCTTTACTTACTACACCTCA
5'生物素标记的PCR扩增引物	BrafPyorBioR	TCCAGACAACTGTTCAAA
PYRO引物	Braf pyroS	GTGATTTTGGTCTAGC

引物	引物名称	引物序列（5'~3'）
PCR扩增引物	BrafPyro F	TCCTTTACTTACTACACCTCA
5'生物素标记的PCR扩增引物	BrafPyorBioR	TCCAGACAACTGTTCAAA
PYRO引物	Braf pyroS	GTGATTTTGGTCTAGC

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