Expressions of leukocyte CD35 and CD11b in the diagnosis of bacterial infection

doi:10.3969/j.issn.1673-8640.2018.10.005

Abstract

Abstract:

Objective To evaluate the roles of leukocyte complement receptor （CR） 1 （CD35） and CR3（CD11b） expressions for the diagnosis of bacterial infection. Methods The mean fluorescence intensities （MFI） of CD35 and CD11b in leukocytes （lymphocytes,monocytes and neutrophils） in 39 patients with bacterial infection and 23 healthy subjects （healthy control group） were determined by flow cytometry. The neutrophil CD35 MFI/lymphocyte CD35 MFI ratio （hereinafter referred to as CD35 ratio） and neutrophil CD11b MFI/lymphocyte CD11b MFI ratio （hereinafter referred to as CD11b ratio） were calculated. CD35 infection index and CD11b infection index were calculated according to the formula： infection index = neutrophil MFI²/（lymphocyte MFI×monocyte MFI）. CD64 MFI was determined,and the CD64 ratio and CD64 infection index were calculated. The performance of each indicator in the diagnosis of bacterial infection was assessed using receiver operating characteristics （ROC） curve. Results The MFI of lymphocyte CD35,CD11b and CD64 in bacterial infection group were lower than those in healthy control group （P<0.000 1,P<0.01,P<0.000 1）. The MFI of neutrophil CD35,CD11b and CD64 in bacterial infection group were higher than those in healthy control group （P<0.000 1,P<0.000 1,P<0.01）. The MFI of monocyte CD11b and CD64 in bacterial infection group were higher than those in healthy control group （P<0.000 1,P<0.01）,and there was no statistical significance for monocyte CD35 MFI between the 2 groups （P>0.05）. In bacterial infection group,CD35 ratio,CD35 infection index,CD11b ratio,CD11b infection index,CD64 ratio and CD64 infection index were higher than those in healthy control group （P<0.000 1）. ROC curve analysis showed that the areas under curves （AUC） of CD35 ratio,CD11b ratio and CD64 ratio had no statistical significance （P>0.05）. The AUC of CD11b infection index was bigger than that of CD64 infection index （P<0.05）,and there was no statistical significance between CD35 infection index and CD64 infection index （P>0.05）. Conclusions CD35 and CD11b could be new markers for the diagnosis of bacterial infection.

Key words: Complement receptor, CD35, CD11b, CD64, Infection, Leukocyte, Flow cytometry

CLC Number:

R446.1

WANG Weiwei, HAO Jun, YUAN Xiangliang, ZHANG Lihua, ZHENG Peiming, FEI Qili, XI Di, SHEN Lisong. Expressions of leukocyte CD35 and CD11b in the diagnosis of bacterial infection[J]. Laboratory Medicine, 2018, 33(10): 894-898.

Figures/Tables 9

References 13

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组别	例数	CD35	CD11b	CD64
细菌感染组	39	2 145±695	5 195±1 547	336±175
正常对照组	23	3 248±692	6 637±1 742	561±161
t值		6.055	3.280	5.131
P值		<0.000 1	<0.01	<0.000 1

组别	例数	CD35	CD11b	CD64
细菌感染组	39	2 145±695	5 195±1 547	336±175
正常对照组	23	3 248±692	6 637±1 742	561±161
t值		6.055	3.280	5.131
P值		<0.000 1	<0.01	<0.000 1

组别	例数	CD35	CD11b	CD64
细菌感染组	39	12 598±5 550	111 649±35 125	3 065±2 751
正常对照组	23	5 895±2 675	28 772±9 475	1 805±476
t值		6.388	13.900	2.791
P值		<0.000 1	<0.000 1	<0.01

组别	例数	CD35	CD11b	CD64
细菌感染组	39	12 598±5 550	111 649±35 125	3 065±2 751
正常对照组	23	5 895±2 675	28 772±9 475	1 805±476
t值		6.388	13.900	2.791
P值		<0.000 1	<0.000 1	<0.01

组别	例数	CD35	CD11b	CD64
细菌感染组	39	18 242±7 073	128 988±28 317	9 516±4 574
正常对照组	23	14 383±4 560	77 163±12 853	6 498±936
t值		1.939	9.839	3.981
P值		>0.05	<0.000 1	<0.001