Abstract:

Objective To compare homogeneous enzyme immunoassay and latex-enhanced immuno-turbidimetric assay for the determination of cholyglycine（CG）,and to provide a reference for selecting appropriate method. Methods The positive rates,precisions and linear ranges of the 2 methods were compared,and then 248 samples were determined by the 2 methods for statistical analysis. Results The positive rates of homogeneous enzyme immunoassay and latex-enhanced immunoturbidimetric assay were 53.23% and 51.21%,and the positive coincidence rate was 97.58%. The coefficients of variation （CV）of within-run precision for high-value serum were 1.13% and 2.65%,and those for low-value serum were 3.52% and 7.51%,respectively. In linear evaluation,the linear regression equation of homogeneous enzyme immunoassay was Y=1.068 2X+0.421 6（r =0.995 7）,and the linear regression equation of latex-enhanced immunoturbidimetric assay was Y=1.016 1X+0.875 2（r =0.997 7）. The relationship between the 2 methods was Y=1.156X+0.543 [coefficient of determination（R²）=0.98]. Conclusions The results of the 2 methods have high consistency,and they have good accuracy,stability and reliability,which meet clinical determination requirements. However,the stability of homogeneous enzyme immunoassay is better than that of latex-enhanced immunoturbidimetric assay.

Key words: Cholyglycine, Homogeneous enzyme immunoassay, Latex-enhanced immunoturbidimetric assay