Analysis of trace elements in rabbit kidney with high-adipose diet by ICP-AES

doi:10.3969/j.issn.1673-8640.2015.08.016

Abstract

Abstract:

Objective To investigate the content changes of Fe, Mn, Cu, Zn, Se, Pb and Cd in rabbit kidney with atherosclerosis (AS). Methods The model of rabbit kidney with AS was reproduced by a high-adipose diet, kidney was obtained, the samples were microwave-digested with miscible liquids of nitric acid and peroxide of hydrogen, and then the Fe, Mn, Cu, Zn, Se, Pb and Cd contents were determined by inductive coupled plasma atomic emission spectrometry (ICP-AES). Results Serum total cholesterol (TC) and Zn, Fe, Se and Cd contents in normal control and high-adipase groups had statistical significance (P<0.05). Cd contents of the 2 groups were significantly higher than that in feed sample (P<0.05). Conclusions Mn, Cu, Zn and Se contents in high-adipose group decline, which may have some relation with the intake of foods and the abnormality of lipoprotein metabolism. Cd accumulates easily in kidney.

Key words: Inductive coupled plasma atomic emission spectrometry, Trace element, Kidney

CLC Number:

Q593.1

WU Yunkai, WANG Hu, WEI Wenmei, GU Zhihong, WANG Yuan. Analysis of trace elements in rabbit kidney with high-adipose diet by ICP-AES[J]. Laboratory Medicine, 2015, 30(8): 841-843.

Figures/Tables 5

References 12

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步骤	升温时间 (min)	温度 (℃)	压力 (kPa)	保持时间 (min)
1	10	120	2.41	10
2	5	165	2.41	5
3	5	180	2.41	15

步骤	升温时间 (min)	温度 (℃)	压力 (kPa)	保持时间 (min)
1	10	120	2.41	10
2	5	165	2.41	5
3	5	180	2.41	15

组别	例数	铁(mg/kg)	锰(mg/kg)	铜(mg/kg)	锌(mg/kg)
正常对照组	10	263.60±36.87	5.52±0.88	16.28±1.03	83.33±8.62
高脂饮食组	13	310.70±117.30^*	5.12±1.29	14.9±1.99	65.25±9.71^*
组别	例数	硒(mg/kg)	铅(mg/kg)	镉(mg/kg)	TC(mmol/L)
正常对照组	10	4.06±0.48	0.273 7±0.190 6	3.50±1.11	1.04±0.70
高脂饮食组	13	3.53±0.58^*	0.326 9±0.248 6	2.66±0.72^*	28.33±1.95^*

组别	例数	铁(mg/kg)	锰(mg/kg)	铜(mg/kg)	锌(mg/kg)
正常对照组	10	263.60±36.87	5.52±0.88	16.28±1.03	83.33±8.62
高脂饮食组	13	310.70±117.30^*	5.12±1.29	14.9±1.99	65.25±9.71^*
组别	例数	硒(mg/kg)	铅(mg/kg)	镉(mg/kg)	TC(mmol/L)
正常对照组	10	4.06±0.48	0.273 7±0.190 6	3.50±1.11	1.04±0.70
高脂饮食组	13	3.53±0.58^*	0.326 9±0.248 6	2.66±0.72^*	28.33±1.95^*

微量元素	基础饲料	高脂饲料
铁	370.90	938.90
锰	171.40	166.20
铜	12.93	11.61
锌	49.97	34.44
硒	0.366 8	0.504 9
铅	0.570 6	0.632 4
镉	0.453 2	0.399 6