The detection and analysis of sperm motion parameters and DNA fragmentation index in asthenozoospermia patients

doi:10.3969/j.issn.1673-8640.2015.05.008

Abstract

Abstract: Objective To study the changes of sperm motion parameters and DNA fragmentation index (DFI) in patients with asthenozoospermia. Methods The routine analysis, semen analyzer activity detection and fluorescence microzooscopy of 41 cases of asthenozoospermia patients(asthenozoospermia group) and 48 cases of normal vitality patients(normal vitality group) were performed. Semen routine parameters(sperm concentration, total number of sperm and sperm activity rate), sperm motion parameters [progressive motility(PR), average path velocity(VAP), curvilinear velocity(VCL), straight line velocity(VSL), linearity(LIN), wobble(WOB), straightness(STR), amplitude of lateral head displacement(ALH), beat-cross frequency(BCF) and mean angular displacement(MAD)] and DFI in patients with asthenozoospermia and normal vitality group were compared. Results The sperm concentration, total number of sperm, sperm activity rate, PR, VAP, VCL, VSL, LIN, WOB, STR, ALH, BCF and MAD of asthenozoospermia group were (69.0±49.1)×10⁶/mL, (203±159)×10⁶, 60.8%±15.7%, 21.1%±8.0%,(10.7±2.8)μm/s, (20.6±4.9)μm/s, (6.3±2.2)μm/s, 29.9%±8.1%,51.4%±5.8%, 57.2%±10.4%, (0.53±0.12)μm, (3.20±0.85)Hz and (14.2±3.2)°, respectively. These parameters were significantly lower than those of normal vitality group[(98.8±38.2)×10⁶/mL(P<0.05),(281±171)×10⁶(P<0.05), 82.0%±7.9%(P<0.01), 45.1%±8.5%(P<0.01), (18.5±3.0)μm/s(P<0.01), (30.7±5.3)μm/s(P<0.01), (12.0±2.3)μm/s (P<0.01), 39.6%±7.0%(P<0.01), 60.5%±5.0%(P<0.01), 65.1%±7.2%(P<0.01), (0.72±0.13)μm (P<0.01), (5.07±0.81)Hz(P<0.01) and (17.8±2.5)°(P<0.01)]. The DFI in asthenozoospermia group(17.9%±12.5%) was significantly higher than that in normal vitality group(8.5±6.4%, P<0.01). Conclusions Besides the performance of low sperm motility, asthenozoospermia is still accompanied by decreasing sperm concentration, total number of sperm and sperm motion parameters and increasing sperm DFI. The change of these parameters may be one of the mechanisms of male infertility caused by asthenozoospermia.

Key words: Sperm motion parameter, DNA fragmentation index, Semen routine parameter, Asthenozoospermia

CLC Number:

R446.11

JIAO Ruibao, YAO Yuyou, TANG Jibin, FENG Hengxiao, FENG Junrong. The detection and analysis of sperm motion parameters and DNA fragmentation index in asthenozoospermia patients[J]. Laboratory Medicine, 2015, 30(5): 442-445.

Figures/Tables 1

References 10

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组别	例数	年龄 (岁)	精子浓度 (×10⁶/mL)	精液量 (mL)	精子总数 (×10⁶)	活动率 (%)
弱精子症组	41	31.7±5.5	69.0±49.1	3.1±1.7	203.0±159.0	60.8±15.7
活力正常组	48	30.1±5.4	98.8±38.2	3.0±1.5	281.0±171.0	82.0±7.9
t值		1.37	3.22	0.36	2.21	7.84
P值		>0.05	<0.05	>0.05	<0.05	<0.01
组别	例数	PR (%)	VAP (μm/s)	VCL (μm/s)	VSL (μm/s)	ALH (μm)
弱精子症组	41	21.1±8.0	10.7±2.8	20.6±4.9	6.3±2.2	0.53±0.12
活力正常组	48	45.1±8.5	18.5±3.0	30.7±5.3	12.0±2.3	0.72±0.13
t值		13.64	12.5	9.35	12.07	6.77
P值		<0.01	<0.01	<0.01	<0.01	<0.01
组别	例数	BCF (Hz)	MAD (°)	LIN (%)	WOB (%)	STR (%)
弱精子症组	41	3.20±0.85	14.2±3.2	29.9±8.1	51.4±5.8	57.2±10.4
活力正常组	48	5.07±0.81	17.8±2.5	39.6±7.0	60.5±5.0	65.1±7.2
t值		10.58	6.11	6.02	7.9	4.09
P值		<0.01	<0.01	<0.01	<0.01	<0.01

组别	例数	年龄 (岁)	精子浓度 (×10⁶/mL)	精液量 (mL)	精子总数 (×10⁶)	活动率 (%)
弱精子症组	41	31.7±5.5	69.0±49.1	3.1±1.7	203.0±159.0	60.8±15.7
活力正常组	48	30.1±5.4	98.8±38.2	3.0±1.5	281.0±171.0	82.0±7.9
t值		1.37	3.22	0.36	2.21	7.84
P值		>0.05	<0.05	>0.05	<0.05	<0.01
组别	例数	PR (%)	VAP (μm/s)	VCL (μm/s)	VSL (μm/s)	ALH (μm)
弱精子症组	41	21.1±8.0	10.7±2.8	20.6±4.9	6.3±2.2	0.53±0.12
活力正常组	48	45.1±8.5	18.5±3.0	30.7±5.3	12.0±2.3	0.72±0.13
t值		13.64	12.5	9.35	12.07	6.77
P值		<0.01	<0.01	<0.01	<0.01	<0.01
组别	例数	BCF (Hz)	MAD (°)	LIN (%)	WOB (%)	STR (%)
弱精子症组	41	3.20±0.85	14.2±3.2	29.9±8.1	51.4±5.8	57.2±10.4
活力正常组	48	5.07±0.81	17.8±2.5	39.6±7.0	60.5±5.0	65.1±7.2
t值		10.58	6.11	6.02	7.9	4.09
P值		<0.01	<0.01	<0.01	<0.01	<0.01

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