The application of adenosine triphosphate bioluminescence assay in the diagnosis of multidrug-resistant Mycobacterium tuberculosis

doi:10.3969/j.issn.1673-8640.2013.12.014

›› 2013, Vol. 28 ›› Issue (12): 1121-1123.DOI: 10.3969/j.issn.1673-8640.2013.12.014

• Orginal Article • Previous Articles Next Articles

The application of adenosine triphosphate bioluminescence assay in the diagnosis of multidrug-resistant Mycobacterium tuberculosis

LIU Jun¹,HU Jiabo¹, PEI Hao², KUAI Shougang², CHEN Liyan³.

1. Institute of Basic Medicine and Medical Technology, Jiangsu University, Jiangsu Zhenjiang 212013, China；2.Wuxi Fifth People′s Hospital, Jiangsu Wuxi 214005, China；3.Wuxi Helie Community Health Service Center, Jiangsu Wuxi 214072, China

Received:2013-01-30 Online:2013-12-01 Published:2013-12-20

Abstract

Abstract:

Objective　 To compare with Roche solid culture method, and to evaluate the feasibility of adenosine triphosphate bioluminescence assay for detecting multidrug-resistant Mycobacterium tuberculosis. Methods　 By Roche solid culture method and adenosine triphosphate bioluminescence assay, 149 clinical isolates of Mycobacterium tuber-culosis were determined and analyzed. Results　 The coincidence rate of adenosine triphosphate bioluminescence assay with Roche solid culture method was 92.6% (138/149), and the difference had no statistical significance (χ²=0.57, P=0.45). The detection time of adenosine triphosphate bioluminescence assay was (6.6±2.1) d, which was faster than that of Roche solid culture method (28 d, t=422.7, P<0.001). Conclusions　 Compared with the conventional detection methods, adenosine triphosphate bioluminescence assay is simple, rapid and accurate. It is helpful for detecting multidrug-resistant tuberculosis patients and controlling the prevalence of Mycobacterium tuberculosis. It is suitable for clinical laboratory.

Key words: Adenosine triphosphate bioluminescence assay, Drug sensitivity test, Multidrug-resistant Mycobacterium tuberculosis

CLC Number:

R446.5

LIU Jun,HU Jiabo, PEI Hao, KUAI Shougang, CHEN Liyan.. The application of adenosine triphosphate bioluminescence assay in the diagnosis of multidrug-resistant Mycobacterium tuberculosis[J]. , 2013, 28(12): 1121-1123.

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The application of adenosine triphosphate bioluminescence assay in the diagnosis of multidrug-resistant Mycobacterium tuberculosis

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