The study of acquired resistant genes against aminoglycosides in extensively drug-resistant Acinetobacter baumannii

doi:10.3969/j.issn.1673-8640.2013.11.010

Abstract

Abstract:

Objective　To investigate the situation of acquired resistant genes against aminoglycosides in extensively drug-resistant (XDR) Acintobacter baumannii (AB) isolated from intensive care unit (ICU) patients. Methods　Bacterial identification and susceptibility tests of AB were performed by phoenix^TM-100 automatic bacterial identification and susceptibility analyzer, and the AB identification was confirmed by gyrA and parC gene amplification sequencing. For the 20 isolates of XDR-AB, 10 aminoglycoside modifying enzyme genes, 6 16S rRNA methylase genes and efflux pump adeB genes were detected by polymerase chain reaction(PCR) and verified and compared by DNA sequencing. Results　Of the 20 isolates of XDR-AB, the detection rates of aac(3)-Ⅰ,aac(6′)-Ⅰb,ant(3″)-Ⅰ and aph(3′)-Ⅰ genes were 90.0%,30.0%,95.0% and 95.0%,respectively.However, aac(3)-Ⅱ,aac(6′)-Ⅰad,aac(6′)-Ⅱ,ant(2″)-Ⅰ,ant(4′)-Ⅰand aph(3′)- Ⅵa genes were not found.In addition,the armA 16S rRNA methylase gene and efflux pump adeB genes existed in all of the 20 isolates, and the detection rates were 100%. Conclusions　All of the 20 isolates of XDR-AB carry not only armA and adeB genes but also a large amount of aac(3)-Ⅰ,ant(3″)-Ⅰand aph(3)-Ⅰgenes.It indicates that the high-level resistance to aminoglycosides in XDR-AB isolated from ICU may be associated with the resistant genes.

Key words: Extensively drug-resistant Acintobacter baumannii, Aminoglycoside modifying enzyme, Acquired resistant gene, Sequencing analysis

CLC Number:

R446.5

WANG Yuyue,SHI Weifeng,ZHOU Jun.. The study of acquired resistant genes against aminoglycosides in extensively drug-resistant Acinetobacter baumannii[J]. , 2013, 28(11): 1008-1011.

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