›› 2013, Vol. 28 ›› Issue (9): 841-844.DOI: 10.3969/j.issn.1673-8640.2013.09.025

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Effects of vasoactive intestinal peptide to the activation of GFAP after focal cerebral ischemia-reperfusion in rats

LIU Xu.   

  1. Department of Clinical Loboratory, the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China
  • Received:2012-08-09 Revised:2013-09-25 Online:2013-09-15 Published:2013-09-25

Abstract:

Objective To investigate the effects of vasoactive intestinal peptide(VIP) on the dynamic changes of glial fibrillary acidic protein(GFAP) after focal cerebral ischemia-reperfusion in vivo and the proliferation of astrocyte, and discuss the neuroprotective effects of VIP. Methods In rats, VIP was given via injection, and middle cerebral artery occlusion (MCAO) model was set up by suture method. The evaluation and screening of rats with successful occlusion of MCAO were performed by neurological testing. The expression of GFAP was detected by immunohistochemistry. Results Compared with the control groups after VIP injection, GFAP expression significantly increased(P<0.05). The existing astrocyte response in the same ischemic area was upregulated. Conclusions VIP injection enhances the expression of GFAP and reduces the degree of cerebral ischemia-reperfusion injury. This study provides the evidence for the neuroprotective effects of VIP in vivo.

Key words: Glial fibrillary acidic protein, Vasoactive intestinal peptide, Cerebral ischemia-reperfusion, Astrocyte

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