The variation of immature platelet fraction in patients with thrombocytopenic diseases

doi:10.3969/j.issn.1673-8640.2013.01.012

Abstract

Abstract: ,Objective　To investigate the variation of immature platelet fraction（IPF)，high-fluorescence intensity of immature platelet fraction（H-IPF)，absolute value of immature platelet（IPF#）and mean side fluorescence intensity of platelet(PLT-X）in patients with thrombocytopenic diseases and their clinical significance in the thrombocytopenic diseases. Methods　The platelet（PLT)，IPF，H-IPF，IPF# and PLT-X of peripheral blood in 86 patients with thrombocytopenic diseases [50 cases of idiopathic thrombocytopenic purpura（ITP)，15 cases of aplastic anemia（AA）and 21 cases of acute leukemia（AL)]，32 patients with myeloproliferative disorders（MPD）[11 cases of chronic myelogenous leukemia（CML)，16 cases of essential thrombocythemia(ET）and 5 cases of polycythemia vera（PV)] and 50 healthy subjects were determined by automatic hematology SYSMEX XE-5000 analyzer. According to the results of PLT，the 50 cases of ITP were classified into ≤30×10⁹/L，(＞30-＜60)×10⁹/L and（60-＜100)×10⁹/L groups，and the results for IPF were compared. Results　The IPF，H-IPF，IPF# and PLT-X in ITP group were（19.8±12.7）%，6.7（4.7-12.3）%，3.1（2.0-12.1）×10⁹/L and（27.8±8.6）ch，respectively. The IPF，H-IPF，IPF# and PLT-X in AA group were（5.6±2.5）%，1.9（0.7～4.0）%，0.8（0.4～1.4）×10⁹/L and（17.3±2.4）ch，respectively. The IPF，H-IPF，IPF# and PLT-X in AL group were（6.3±3.4）%，2.1（1.2～4.1）%，2.4（1.5～3.2）×10⁹/L and（18.7±3.0)ch，respectively. The IPF，H-IPF，IPF# and PLT-X in MPD group were（3.1±1.6）%，0.9（0.7～1.4）%，19.2（14.0～22.5）×10⁹/L and（16.9±2.3)ch，respectively. The IPF，H-IPF，IPF# and PLT-X in healthy subject group were（4.1±1.3）%，1.2（1.0～1.7）%，9.3（7.4～12.1）×10⁹/L and（18.4±1.5）ch，respectively. Compared with the AA，AL，MPD groups and healthy subject group，the IPF，H-IPF and PLT-X of ITP group wrer higher（P＜0.05)，and the difference in the IPF# between ITP group and the other 3 groups was significant（P＜0.05)，but there was no statistical significance in the IPF# between ITP group and healthy subject group（P＞0.05). There was no statistical significance for IPF in the different groups of ITP（P＞0.05）.

Key words: Immature platelet fraction, Thrombocytopenic disease, Idiopathic thrombocytopenic purpura

JIANG Weiyan，JIANG Minghua，WU Yizhong，ZHANG Zhaohua，CHEN Xiaojian. The variation of immature platelet fraction in patients with thrombocytopenic diseases[J]. , 2013, 28(1): 47-50.

References

,[1]张之南，沈悌.血液病诊疗及疗效标准[M].北京：科学出版社，2007:172.
[2]Jung H，Jeon HK，Kim HJ，et al. Immature platelet fraction：establishment of a reference interval and diagnostic measure for thrombocytopenia [J]. Korean J Lab Med，2010，30（5)：451-459.
[3]Albanyan A，Murphy MF，Wilcock M，et al. Changes in the immature platelet fraction within ageing platelet concentrates [J]. J Thromb Haemost，2008，6（12)：2213-2215.
[4]孙明艳，姜晓萍，陈立君，等.急性早幼粒细胞白血病患者网织血小板的检测与分析[J]. 血栓与止血学，2009，15（3）：128-130.
[5]王贤，张葵.网织血小板检测在血小板减少症中的临床价值[J].中华实用诊断与治疗杂志，2008，22（11）：835-837.
[6]Pons I，Monteagudo M，Lucchetti G，et al. Correlation between immature platelet fraction and reticulated platelets. Usefulness in the etiology diagnosis of thrombocytopenia[J]. Eur J Haematol，2010，85(2)：158-163.
[7]孙德华，王前，郑磊，等.网织血小板与血常规中血小板相关参数之间的相关性[J]. 血栓与止血学，2008，14（4）：171-174.
[8]Briggs C，Kunka S，Hart D，et al. Assessment of an immature platelet fraction（IPF）in peripheral thrombocytopenia[J]. Br J Haematol，2004，126(1)：93-99.
[9]Abe Y，Wada H，Tomatsu H，et al. A simple technique to determine thrombopoiesis level using immature platelet fraction（IPF）[J]. Thromb Res，2006，118(4)：463-469.
[10]任春云，武锦彪，郭希超. 血液分析仪检测网织血小板比率在血小板减少性疾病中的应用[J].临床检验杂志，2010，28（5）：343-344.
[11]Yamaoka G，Kubota Y，Nomura T，et al. The immature platelet fraction is a useful marker for predicting the timing of platelet recovery in patients with cancer after chemotherapy and hematopoietic stem cell transplantation[J]. Int J Lab Hematol，2010，32(6 Pt 1)：e208-e216.
[12]Abe Y，Wada H，Sakakura M，et al. Usefulness of fully automated measurement of reticulated platelets using whole blood [J]. Clin Appl Thromb Hemost，2005，11(3)：263-270.