CRISPR-Cas13a系统在基因编辑和分子诊断领域中的研究进展

doi:10.3969/j.issn.1673-8640.2022.01.020

检验医学 ›› 2022, Vol. 37 ›› Issue (1): 97-100.DOI: 10.3969/j.issn.1673-8640.2022.01.020

CRISPR-Cas13a系统在基因编辑和分子诊断领域中的研究进展

陈佳灵, 黄颖而, 邓昊, 吴鸿峰, 张婧, 赵奕君, 罗佳豪, 郝文波()

南方医科大学检验与生物技术学院抗体工程研究所,广东广州 510515

收稿日期:2020-08-04 修回日期:2020-09-14 出版日期:2022-01-30 发布日期:2022-03-07
通讯作者: 郝文波
作者简介:郝文波,E-mail： haowa@126.com。
陈佳灵,女,1998年生,学士,主要从事临床免疫学相关研究工作。
基金资助:
国家自然科学基金资助项目(31672536);南方医科大学2020年度大学生创新创业训练计划资助项目(S202012121080)

Research progress on a gene editing and molecular diagnostic system CRISPR-Cas13a

CHEN Jialing, HUANG Yinger, DENG Hao, WU Hongfeng, ZHANG Jing, ZHAO Yijun, LUO Jiahao, HAO Wenbo()

Institute of Antibody Engineering,School of Laboratory Medicine and Biotechnology,Southern Medical University,Guangzhou 510515,Guangdong,China

Received:2020-08-04 Revised:2020-09-14 Online:2022-01-30 Published:2022-03-07
Contact: HAO Wenbo

摘要/Abstract

摘要：

成簇的规律间隔的短回文重复序列（CRISPR）-CRISPR相关蛋白（Cas）系统是一个强大的基因编辑工具。相对于Cas9,Cas13a可靶向多基因转录产物,从而调控基因功能表达,填补了Cas9仅限于DNA水平的编辑及脱靶效应等缺陷。不仅如此,利用Cas13a靶向RNA的特性,该系统被成功地改造成下一代核酸诊断工具。文章概述了CRISPR-Cas13系统在基因编辑及分子诊断领域的最新研究进展,并对该系统的应用前景进行了展望。

关键词: 成簇的规律间隔的短回文重复序列, 成簇的规律间隔的短回文重复序列相关蛋白13a, 附带切割, 基因编辑, 特异性高灵敏度酶报告系统

Abstract:

Clustered regularly interspaced short palindromic repeats（CRISPR）-CRISPR-associated protein（Cas） system is a powerful tool for gene editing. Compared with Cas9,Cas13a targeted multiple gene transcription products and regulated gene function expression,filling Cas9 with defects such as editing and missing effect only limited to DNA level. Moreover,using the characteristics of Cas13a targeting RNA,the system has been successfully transformed into the next generation of nucleic acid diagnostic tools. In this review,the latest research progress of CRISPR-Cas13 system in the field of gene editing and molecular diagnosis is summarized,and the application prospect of this system has prospected.

Key words: Clustered regularly interspaced short palindromic repeat, Clustered regularly interspaced short palindromic repeat-associated protein 13a, Incidental cleavage, Gene editing, Specific high-sensitivity enzymatic reporter unlocking

中图分类号:

R446.1

陈佳灵, 黄颖而, 邓昊, 吴鸿峰, 张婧, 赵奕君, 罗佳豪, 郝文波. CRISPR-Cas13a系统在基因编辑和分子诊断领域中的研究进展[J]. 检验医学, 2022, 37(1): 97-100.

CHEN Jialing, HUANG Yinger, DENG Hao, WU Hongfeng, ZHANG Jing, ZHAO Yijun, LUO Jiahao, HAO Wenbo. Research progress on a gene editing and molecular diagnostic system CRISPR-Cas13a[J]. Laboratory Medicine, 2022, 37(1): 97-100.

图/表 2

参考文献 23

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CRISPR-Cas13a系统在基因编辑和分子诊断领域中的研究进展

Research progress on a gene editing and molecular diagnostic system CRISPR-Cas13a

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