寄生虫病的实验室检查方法

doi:10.3969/j.issn.1673-8640.2021.010.002

摘要/Abstract

摘要：

寄生虫病的实验室检查包括病原学检查、免疫学检查和分子生物学检查,在寄生虫病的诊断中起着重要的作用。传统的寄生虫病原学检查虽然直观、准确,但有一定的局限性。随着医疗技术的飞速发展,免疫学检查和分子生物学检查等敏感性和特异性更高,重复性更好,使寄生虫实验室检测效率有了较大的提升。文章就目前寄生虫病的实验室检测技术进行梳理和介绍,以期为寄生虫病的诊治提供参考。

关键词: 寄生虫, 寄生虫病, 实验室检查

Abstract:

Clinical laboratory examinations for parasitic diseases play an important role in the diagnosis of parasitic diseases. Clinical laboratory examinations for parasitic diseases include pathogenic examinations,immunological examinations and molecular biological examinations. Although the traditional parasitological examination is intuitive and accurate,it has some limitations. With the rapid development of medical technologies,parasite laboratory examination technology has also made great progress. Immunological examinations and molecular biological examinations have good sensitivities,specificities and repeatabilities. This review summarizes and introduces clinical laboratory examination technologies for parasitic diseases.

Key words: Parasite, Parasitic disease, Clinical laboratory examination

中图分类号:

R446.1

蔡祺, 王剑飚. 寄生虫病的实验室检查方法[J]. 检验医学, 2021, 36(10): 1001-1007.

CAI Qi, WANG Jianbiao. Clinical laboratory examinations for parasitic diseases[J]. Laboratory Medicine, 2021, 36(10): 1001-1007.

参考文献 34

[1]	刘艳辉. 寄生虫感染实验室检验[J]. 中外医疗, 2009, 28(35):156-157.
[2]	王钊, 王好, 钱爱东, 等. 新型免疫检测方法在五种寄生虫疾病诊断中的应用进展[J]. 中国兽药杂志, 2015, 49(7):63-69.
[3]	邹洋, 李晶晶, 贾永根, 等. 寄生虫病分子生物学诊断进展[J]. 热带医学杂志, 2018, 18(6):830-834.
[4]	王光雷. 寄生虫病的实验室诊断技术[J]. 草食家畜, 2013, 34(3):39-43.
[5]	NORGAN A P, ARGUELLO H E, SLOAN L M, et al. A method for reducing the sloughing of thick blood films for malaria diagnosis[J]. Malar J, 2013, 12:231. DOI URL
[6]	MOMILOVI S, CANTACESSI C, ARSI-ARSENIJEVI V, et al. Rapid diagnosis of parasitic diseases:current scenario and future needs[J]. Clin Microbiol Infect, 2019, 25(3):290-309. DOI URL
[7]	石锋, 杨益, 汪俊云, 等. 快速检测美洲钩虫特异抗体胶体金免疫层析试条方法的建立与效果评价[J]. 中国寄生虫学与寄生虫病杂志, 2018, 36(2):119-123.
[8]	GAO C H, WANG J Y, SHI F, et al. Field evaluation of an immunochromatographic test for diagnosis of cystic and alveolar echinococcosis[J]. Parasit Vectors, 2018, 11(1):311. DOI URL
[9]	TACHIBANA H, KAKINO A, KAZAMA M, et al. Development of a sensitive immunochromatographic kit using fluorescent silica nanoparticles for rapid serodiagnosis of amebiasis[J]. Parasitology, 2018, 145(14):1890-1895. DOI URL
[10]	孟庆东, 王燕, 梁焕坤, 等. 恶性疟与间日疟双标记时间分辨免疫荧光检测试剂盒的研制[J]. 中国病原生物学杂志, 2017, 12(3):258-261.
[11]	YLLMAZ A, USLU H. Examination of Giardia intestinalis with direct microscopy and direct fluorescent antibody in patients with diarrhea[J]. Turkiye Parazitol Derg, 2020, 44(4):187-190. DOI URL
[12]	BHALLA N, JOLLY P, FORMISANO N, et al. Introduction to biosensors[J]. Essays Biochem, 2016, 60(1):1-8. DOI URL
[13]	朱逢龙, 廉晓丽, 季亚楠, 等. 纳米金在医学寄生虫学应用中的研究进展[J]. 中国病原生物学杂志, 2019, 14(9):1108-1111.
[14]	张玉娟, 刘苗, 刘培, 等. 应用弓形虫截短型SAG1纳米金免疫传感器检测弓形虫抗体的研究[J]. 中国病原生物学杂志, 2019, 14(6):661-664.
[15]	付益修, 孔庆明, 郑斌, 等. 基于纳米抗体和石墨烯/金复合材料的免疫传感器检测日本血吸虫循环抗原的研究[J]. 寄生虫与医学昆虫学报, 2017, 24(2):70-78.
[16]	BEYLS N, COGNET O, STAHL J P, et al. Serodiagnosis of extraintestinal amebiasis:retrospective evaluation of the diagnostic performance of the Bordier® ELISA kit[J]. Korean J Parasitol, 2018, 56(1):71-74. DOI URL
[17]	LONGLEY R J, WHITE M T, TAKASHIMA E, et al. Development and validation of serological markers for detecting recent Plasmodium vivax infection[J]. Nat Med, 2020, 26(5):741-749. DOI URL
[18]	DIAS D S, RIBEIRO P A F, SALLES B C S, et al. Serological diagnosis and prognostic of tegumentary and visceral leishmaniasis using a conserved Leishmania hypothetical protein[J]. Parasitol Int, 2018, 67(3):344-350. DOI URL
[19]	ZHU H, ZHANG H, XU Y, et al. PCR past,present and future[J]. Biotechniques, 2020, 69(4):317-325. DOI URL
[20]	张飞燕, 赵玲, 金洁, 等. 多重PCR技术在实验动物病原检测中的应用[J]. 中国比较医学杂志, 2018, 28(10):111-116.
[21]	POMARI E, PIUBELLI C, PERANDIN F, et al. Digital PCR:a new technology for diagnosis of parasitic infections[J]. Clin Microbiol Infect, 2019, 25(12):1510-1516. DOI URL
[22]	许静, 夏超明. 寄生虫病核酸诊断的研究进展[J]. 寄生虫与医学昆虫学报, 2007, 14(3):188-191.
[23]	王莹, 贡桑曲珍, 庞华胜, 等. 基于游离DNA的棘球蚴病诊断标志物的筛选和应用初探[J]. 中国病原生物学杂志, 2020, 15(6):674-677.
[24]	WU Y, TIAN X, SONG N, et al. Application of quantitative PCR in the diagnosis and evaluating treatment efficacy of leishmaniasis[J]. Front Cell Infect Microbiol, 2020, 10:581639. DOI URL
[25]	SEILIE A M, CHANG M, HANRON A E, et al. Beyond blood smears:qualification of Plasmodium 18S rRNA as a biomarker for controlled human malaria infections[J]. Am J Trop Med Hyg, 2019, 100(6):1466-1476. DOI URL
[26]	NOTOMI T, OKAYAMA H, MASUBUCHI H, et al. Loop-mediated isothermal amplification of DNA[J]. Nucleic Acids Res, 2000, 28(12):E63. DOI URL
[27]	COMPTON J. Nucleic acid sequence-based amplification[J]. Nature, 1991, 350(6313):91-92. DOI URL
[28]	朱志伟, 赵金红. 环介导等温扩增技术在寄生虫检测中的应用进展[J]. 热带病与寄生虫学, 2020, 18(4):234-238.
[29]	ORDÓÑEZ D, FERNÁNDEZ-SOTO P, FERNÁNDEZ-MARTÍN A M, et al. A Trypanosoma cruzi genome tandem repetitive satellite DNA sequence as a molecular marker for a LAMP assay for diagnosing chagas' disease[J]. Dis Markers, 2020, 2020:8074314.
[30]	GRAB D J, NIKOLSKAIA O V, COURTIOUX B, et al. Using detergent-enhanced LAMP for African trypanosome detection in human cerebrospinal fluid and implications for disease staging[J]. PLoS Negl Trop Dis, 2019, 13(8):e0007631.
[31]	CHAOUCH M, AOUN K, BEN OTHMAN S, et al. Development and assessment of Leishmania major and Leishmania tropica specific loop-mediated isothermal amplification assays for the diagnosis of cutaneous leishmaniasis in Tunisia[J]. Am J Trop Med Hyg, 2019, 101(1):101-107. DOI URL
[32]	MORRIS U, AYDIN-SCHMIDT B. Performance and application of commercially available loop-mediated isothermal amplification(LAMP) kits in malaria endemic and non-endemic settings[J]. Diagnostics(Basel), 2021, 11(2):336.
[33]	吴忠道, 宋兰桂, 刘超, 等. 我国寄生虫病防控面临的新挑战与新要求[J]. 热带医学杂志, 2019, 19(1):1-3.
[34]	江莉, 黄浦雁, 吴寰宇, 等. 我国疾病预防控制机构国家认证认可实验室寄生虫(病)检测能力的现状分析[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(2):224-233.