关键词: 抗菌肽, 家蝇胚胎细胞, 高三尖杉酯碱, 人髓样白血病细胞K562, 人脐静脉血管内皮细胞

Abstract:

Objective To study the inhibition of antibacterial peptide from embryonic cells of Musca domestica and homoharringtonine（HHT） on human myeloid leukemia cell K562. Methods The embryonic cells of Musca domestica were induced using lipopolysaccharide. Antibacterial peptide was extracted and prepared 80,160,320 and 640 μg/mL level groups by phosphate-buffered saline（PBS）. The medium only adding PBS was used as negative control group. The cell cycle and the cell apoptosis ratio of K562 were determined by flow cytometry. The K562 and human umbilical vein vascular endothelial cells during logarithmic growth phase were prepared as HTT and antibacterial peptide groups. K562 and human umbilical vein vascular endothelial cells without treatment were used as normal control group. The effective inhibition ratios of HHT and antibacterial peptide on K562 and human umbilical vein vascular endothelial cells were determined by flow cytometry. Results Compared with negative control group,the G₀/G₁ of K562 was increased,the S was decreased,and the proliferation index was decreased（P<0.05）. Compared with negative control group,the cell apoptosis ratio of K562 in various level groups had no statistical significance（P>0.05）. The effective inhibition ratios of HHT group and antibacterial peptide group on K562 were increased and had statistical significance compared with normal control group（P<0.05）. The effective inhibition ratio in HHT group on human umbilical vein vascular endothelial cells was higher than those in normal control and antibacterial peptide groups（P<0.05）. Conclusions Antibacterial peptide from embryonic cells of Musca domestica can inhibit tumor cells effectively,but no damage to normal cells.

Key words: Antibacterial peptide, Musca domestica embryonic cell, Homoharringtonine, Human myeloid leukemia cell K562, Human umbilical vein vascular endothelial cell