Table of Content

01 January 2001, Volume 16 Issue 01

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论文

Analysis of serum autoantibodies in 385 cases of malignancies

2001, 16(01):  5-7. 

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Objective To study the positive rates of autoantibodies in thepatients with malignancies as well as the locations of their target antigens in cells and identify the essence of their target antigens. Methods Sera of 385 malignancy patients as well as 140 healthy controls were detected with indirect immunofluorescence (IIF) method using antigens extracted from human epithelial cells (Hep-2). Results We detected 95 patients' sera with positive IIF results with westernblotting method. There are significant differences in positive rates, fluorescent patterns, and location of autoantigens in cells between concer group and health group(P＜0.05). Although with age growing, both have increasingly higher positive rates, concer group are much higher than those of control in every age group(P＜0.01). There is no significant difference among different malignancy groups(P＞0.05). Among 95 patients' sera with positive IIF results,90 of them were objected one or more positive bands. A few bands were confirmed as several known autoantibodies usually appearing in some autoimmune diseases according to standards provided by the kit, which included PM-SCL(2), Ku(7), CENP(5), M2(9)， RNP(4)， SS-A(7)， Jo-1(1), SS-A/SS-B(5), Rib-P-Protein(4). The autoantigen of most other bands were still unknown. Conclusion Dispite of age-related, autoantibodies in malignancy patients are malignancy-related. They are different from those in healthy people as well as patients with autoimmune disease. Further efforts should be made to identify the target antigens as well as their biological role and clinical significance.

Seroimmunological analysis of 28 multiple myeloma cases

2001, 16(01):  8-912. 

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Objective To study the seroimmunological features of mutiplemyeloma(MM). Methods 28 serum samples of MM patients were analyzed by agarose gel electrophoresis and electrophoretic scanning, quantitative measurement of serum protein and immunoglubulins, and immunofixation electrophoresis. Results M bands appeared in 96.4%serum sample, in which IgG、 IgM appeared in γ zone and IgA appeared in β zone. The figure of immunofixation electrophoresis varied from types to types of MM, IgG, IgA, IgM and free light chain accounted for 46.4%, 21.4%, 17.9%and 10.7%respectively in all of the M protein. By classification of the light chain types, M proteins of IgG and IgM were mainly κ type, and the light chain types were λ type. Part of the concentration of immonoglobulin which contains monoclonal component increased significantly. Conclusions An overall consideration of the laboratory results from above methods is helpful in making a more accurate laboratory diagnosis for patients with multiple myeloma.

The screen of plasmid-mediated β-lactamases in Escherichia coli and Klebsiella pneumoniae

2001, 16(01):  10-12. 

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Objective To study distribution,phenotypes and detection methodsof plasmid-mediated β-lactamases in Escherichia coli and Klebsiella pneumoniae.Methods Using routine antibiotics susceptibility tests for screening,extended spectrum β-lactamases (ESBL) confirm test for detecting ESBL,and cefoxitin three-dimension test for detecting plasmid-mediated AmpCs.Result Among 341 clinical isolates of Escherichia coli and Klebsiella pneumoniae,there are 164 strains (48.1%) showed MIC of cefpodoxime＞1 μg/ml by routine susceptibility test;102 strains of them were proceeded further detection,64 strains produced ESBL,only 6 strains were plasmidincode AmpCs.Conclusion The result indicated that ESBL (30.2%) were more than plasmid-incode AmpCs (2.8%) in plasmid-mediated β-lactamases in Escherichia coli and Klebsiella pneumoniae.

Rapid detection of bacteriuria with microscopic examination

2001, 16(01):  13-14. 

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Objective To develop a rapid method for the detection ofbacteriuria. Methods 10 μl of urine sample was smeared on the slide, examined by microscopy after Gram stain; 10 ul of urine sample was examined by cul-ture. Results 100 samples showed positive in both microscopic examination (≥2 bacteria per oil immersion field) and cultures (colony counts of ≥105 CFU/ml); 1047 samples both negative; six samples microscopic examination positive and cultures negative; two samples microscopic examination negative and cultures positive. So the resolved sensitivity and specificity of the microscopic examination are 98.0%and 99.4%respectively; Positive predictive value and negative predictive value are 94.3%and 99.8%respectively. Conclusion The method for detecting significant bacteriuria by microscopic examination with 10 μl sample is rapid, effective and simple.

The Significance of the change of plasma protein C in patients having oral anticoagulant after mechanical prosthetic valves operation

2001, 16(01):  15-17. 

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Objective To investigate the changes of plasma protein C (PC) inpatients having oral warfarin after mechanical prosthetic valves operation and its relationship with the changes of other coagulation factors. Methods Plasma PC was measured by ELISA.FX:C,and PT(INR) were measured by one stage method. Results The concentration of PC of control group and oral warfarin group were(5.0±0.64)mg/L,(2.54±2.18)mg/L respectively. There was significant difference between the two groups. These concentrations of PC were(4.02±3.60)mg/L,(2.52±1.56)mg/L and (2.40±1.66)mg/L,in patients who have warfarin for 1～6 months, 7～24 months and 25～132 months respectively. The change of PC have no correlation with FX、PT and INR. Conclusion The patients having oral warfarin with mechanical prosthetic valves operation, had a lower concentration of PC, and it related with the time of having warfarin, there was great individual variation, in the change of plasma PC. Surveillance of PC may have important clinical significance in the early stage of anticoagulant treatment.