Laboratory Medicine

The establishment of the method to examine the anti-Sa antibody by Western-blot

2001, 16(02): 68-69.

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Objective To establish the method to examine the anti-Sa antibody and study its value in diagnosing rheumatoid arthritis (RA). Method The optimal conditions to examine the anti-Sa antibody by Western blot have been selected through three groups of complete cross tests. We examined the anti-Sa antibody in the sera of patients with RA or other autoimmunity diseases and healthy people. The RF was examined by immuno latex agglutination. Results The sensitivity of anti-Sa antibody to RA is 42.7%and its specificity is 100%, the sensitivity of RF is 72.0%and its specificity is 79.2%. Conclusion The specificity of the anti-Sa antibody is higher than that of RF, so the diagnostic value of anti-Sa antibody was better than that of RF.

Megaloblastic anemia characterized by granulocytic series with giants——with report of 6 cases

LU Xingguo;CHENG Qiaoqun;ANG Zhongguo

2001, 16(02): 70-72.

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ObjectiveTo report 6 cases of megaloblastic anemia (MA) characterized by granulocytic series with extreme or significant hypercellularity and giants. MethodThe characteristics of usual clinic, hemogram and myelogram of MA characterized by extreme or significant hypercellualrity and giants were analyzed with morphologic examination. ResultsIn these cases, there were mostly significantly decreased WBC and Plt in peripheral blood and granulocytic series with extreme or significant hypercellualrity and giants but erythrocytic series with not significant hypercellularity and decreased cell quantity indeed in bone marrow. There was erythrocytic series mostly with slightly or intermediately megaloblastic changes. There were rubriblasts and prorubricytes with less or no typical megaloblastic changes which could be seen in general MA. There was significant therapy effect with vitamin B12 or vitamen B12 and folic acid as general MA. ConclusionThe MA characterized by granulocytic series with extreme or significant hypercellularity and giants was a special type of MA. It is easy to diagnose incorrectly and difficult to diagnose due to deficient cognition to it.

Serum tissue factor level and mRNA detection in patients with gastrointestinal tumor

2001, 16(02): 73-757.

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Objective　 To investigate the serum level of tissue factor (TF) and mRNA expression in monocyte from patients with gastrointestinal tumor. Methods The TF level was determined in 32 gastric cancers, 45 colorectal carcinomas, 30 normal persons were determinated with ELISA, RT-PCR was used to detect expression of mRNA in monocyte. Results The high serum TF level was found in gastrointestinal tumor. TF mRNA in monocyte can be detected in most of cancer patients whereas it is undetectable in normals. Conclusions Expression of TF mRNA in monocyte probably accounts for high level of TF level in serum, TF value and mRNA expression can be a useful tool for early diagnosis and studying pathological processes.

The predictive value of a si ngle beta human chorionic gonadotropin determination in pregnancies achieved by assisted reproductive technology

YE Aiqing;XIE Xinyou

2001, 16(02): 76-78.

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Objective To study whether a single s erum β-hCG determination in pregnancies ach ieved by assist ed reproductive technologies(ART) can accurately predict pregna ncy viability and inviable pregnancies, multiple gestation. Method Fo urty-nine consecutive succe ssful ART pregnancies between November 1998 and February 2000 were studied retrospectively. Fourty-six subjects had a serum β-hCG determinetion 14 day s after ET were analyzed. Results 36 of the 46(78.3%) were found to be viable. The mean β-hCG value of the nonviable was(89±24) U/L, the viable was(472±45)U/L，statistically significant differences were fou nd between thes e two groups, P＜0.05. According to the cut off v alue of a single β-hCG＞100 U/L in distinguishing viable from nonviable pregnancies achieved, the sensitivity was 97%, specificity 80%,the positive predictive value 95%,the negative p redictive value 89%. Ten of the 46 were multiple gestations, the mean β-hCG o f the multiple ge stations ［(656±70) U/L］ was significantly different from that o f the singleton pregnancies ［(288±20)U/L］.The probability of viable, and fu rthermore multiple p regnancies increase when the value of β-hCG goes up. Conclusion A single early serum β-hCG determinaton 14 alays after ET is clinically valuab le in predicting p regnancy outcome achieved by ART. It will help the gynecologists to take some m easurements in timely. Early reassuring tests may reduce an xiety of the couple.

Usefulness of calibrators in standardization of enzyme ac ti vities by different analyzer/reagent systems

2001, 16(02): 79-808.

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Objective To study the effects of calibra tors in unifying the results of di fferent analyzer/reagent systems. Methods The ac tivities of ALT, AST, CK，GGT, LD in 150 and ALP in 100 fresh sera were measured by using four an alyzer/reagent systems , and the mean values of different systems were compared between, before and afte r calibration with calibrators. ResultsThe d ifferences among the results of fou r systems were observed before calibration. The coefficient s of variation(CVs) are all more than or cqual to 7.0%,more r emarkably these of ALP and GGT are 27.0%and 17.4%respectively. After calibr ation, the differences a re decreased clearly and the CVs are all lower than 5%except that ALP and GGT are 6.6%and 5.1%respectively. ConclusionEnzym e calibrators could be useful in standardizing the results of different analyzer/reagent systems.

A matrix effect-free bleomycin assay in detecting non-protein binding iron of newborn cord plasma by a standard plasma

2001, 16(02): 81-83.

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Objective To establish a practical method of detecting cord plasma nonprotein bound iron(NPBI). M ethods Of 20 cord plasma samples,10 of mormal, 10 of asphyxial newborns, respectively, their NPBI were investigated by traditional and corrected blo emycin assay. Their standard curves, recovery rates and t-test statistical results were compared. Results We found the cord plasma NPBI were lower detected by tra ditional bleomycin assay in normal and asphyxial newborns, their recovery rate was only about 32%. There is an obviousely matrix effect. Though the NPBI of normal and asphyxial ne wborns had still significant difference by t-test,the NPBI values were so l ow th at it's easily disturbed and difficult to practice on clinical use. The detecte d results were improved by matrix effec-free bleomycin assay. Their rec overy rat es were reached about 101%and the NPBI values had statistical significant d iffe rence between normal and asphyxial newborn. Conclusion The matri x effec-free bleo mycin assay is a stable, real and better method to estimate the concentration of plasma NPBI practically on clinical use.

CRP、PA、IL-6 in children with acute respiratory infection

2001, 16(02): 84-85.

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Study on detecting assay for endoxin and β-1,3-D-glucan in serum

WEN Ping

2001, 16(02): 96-97.

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Clinical isolation results and antibiotic resistant patterns of imipenem resistant Ps.aeruginosa