Determination of CD64 index by flow cytometry in the diagnosis of bacterial infection

doi:10.3969/j.issn.1673-8640.2017.06.016

Abstract

Abstract:

Objective To establish a determination of CD64 index by flow cytometry （FCM）,and to evaluate its value in the diagnosis of bacterial infection. Methods Polymorphonuclear（PMN）CD64 mean fluorescence intensities （MFI） in 42 healthy controls,41 patients with bacterial infection and 50 patients with non-bacterial infection were determined by FCM,and CD64 indices were calculated. The level of C-reactive protein （CRP）,white blood cell （WBC） count and the percentage of neutrophils（Neu%） were analyzed simultaneously. Receiver operating characteristic （ROC） curve was used to evaluate the value of CD64 index in the diagnosis of bacterial infection. Results CD64 index in bacterial infection group [3.69（2.69-5.25）] was higher than those in healthy control group [0.79（0.40-1.31）,P<0.01] and non-bacterial infection group [0.52（0.40-0.93）,P<0.01]. According to ROC curves,the areas under the curves in turn were CD64 index （0.964）>CRP level（0.931）> Neu% （0.815）>WBC count（0.678）,and the optimal cut-off value of CD64 index was 1.84. The diagnostic sensitivity and specificity were 87.8% and 95.7%. Conclusions Compared with traditional parameters,CD64 index by FCM with high sensitivity and specificity could be used in the diagnosis of bacterial infection.

Key words: CD64 index, Bacterial infection, Flow cytometry

CLC Number:

R446.1

YAN Peiyi, ZHANG Ji, ZOU Yuhan, JIN Shu. Determination of CD64 index by flow cytometry in the diagnosis of bacterial infection[J]. Laboratory Medicine, 2017, 32(6): 514-519.

Figures/Tables 5

References 26

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组别	例数	PMN CD64 MFI	CD64指数	WBC计数（×10⁹/L）	Neu%	CRP（mg/L）
健康对照组	42	37.67 （26.88~49.72）	0.79 （0.40~1.31）	5.90 （5.40~7.40）	55.95 （53.75~58.93）	3.34 （3.00~6.31）
细菌感染组	41	113.03 （91.76~164.36）^*#	3.69 （2.69~5.25）^*#	7.90 （6.30~10.90）^*#	77.40 （63.80~86.00）^*#	45.50 （30.50~83.25）^*#
非感染组	50	40.18 （29.22~51.09）	0.52 （0.40~0.93）	6.40 （5.38~8.70）	68.20 （62.25~72.43）	5.08 （3.34~9.93）

组别	例数	PMN CD64 MFI	CD64指数	WBC计数（×10⁹/L）	Neu%	CRP（mg/L）
健康对照组	42	37.67 （26.88~49.72）	0.79 （0.40~1.31）	5.90 （5.40~7.40）	55.95 （53.75~58.93）	3.34 （3.00~6.31）
细菌感染组	41	113.03 （91.76~164.36）^*#	3.69 （2.69~5.25）^*#	7.90 （6.30~10.90）^*#	77.40 （63.80~86.00）^*#	45.50 （30.50~83.25）^*#
非感染组	50	40.18 （29.22~51.09）	0.52 （0.40~0.93）	6.40 （5.38~8.70）	68.20 （62.25~72.43）	5.08 （3.34~9.93）

项目	最佳阈值	ROC曲线下面积（95%可信区间）	敏感性（%）	特异性（%）	约登指数
CD64指数	1.84	0.964（0.930~0.997）	87.8	95.7	0.835
CRP	16.00	0.931（0.882~0.980）	85.4	93.5	0.789
Neu%	72.30	0.815（0.731~0.899）	65.9	87.0	0.529
WBC计数	6.60	0.678（0.572~0.785）	73.2	58.7	0.319

项目	最佳阈值	ROC曲线下面积（95%可信区间）	敏感性（%）	特异性（%）	约登指数
CD64指数	1.84	0.964（0.930~0.997）	87.8	95.7	0.835
CRP	16.00	0.931（0.882~0.980）	85.4	93.5	0.789
Neu%	72.30	0.815（0.731~0.899）	65.9	87.0	0.529
WBC计数	6.60	0.678（0.572~0.785）	73.2	58.7	0.319

组别	例数	PMN CD64 MFI	CD64指数	WBC计数（×10⁹/L）	Neu%	CRP（mg/L）
革兰阳性菌组	17	109.65 （61.53 ~162.19）	3.69（2.62~5.10）	8.00 （6.25~12.45）	82.10（66.15~85.90）	46.30（32.25~67.60）
革兰阴性菌组	24	118.46（97.13~166.53）	3.95（2.84~5.58）	7.80 （6.25~10.65）	76.45（68.05~88.53）	43.20（14.80~95.75）