Roles of serum HYAL1 and HA in the auxiliary diagnosis and therapeutic monitoring of colorectal cancer

doi:10.3969/j.issn.1673-8640.2023.04.003

Abstract

Abstract:

Objective To investigate the levels of serum hyaluronidase 1（HYAL1） and hyaluronan（HA） and their clinical values in patients with colorectal cancer. Methods Totally，51 healthy subjects（healthy control group），46 patients with colorectal benign lesions（benign lesion group） and 75 colorectal cancer patients（colorectal cancer group） from Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine were enrolled. The clinical data were collected，and serum HYAL1 and HA levels were determined. Serum HYAL1 and HA levels were determined in 41 patients with colorectal cancer after surgery. Pearson correlation analysis was used to evaluate the correlation among all the indicators. Receiver operating characteristic（ROC） curve was used to evaluate the efficacy of single and combined determination of serum HYAL1，HA，carcinoembryonic antigen（CEA） and carbohydrate antigen 19-9（CA19-9） in the diagnosis of colorectal cancer. Results Serum HYAL1 levels in healthy control group，benign lesion group and colorectal cancer group were increased successively（P<0.000 1）. The serum HA level in colorectal cancer group was higher than that in healthy control group （P<0.000 1），but there was no statistical significance in serum HA level between benign lesion group and healthy control group，or between benign lesion group and colorectal cancer group（P>0.05）. Pearson correlation analysis showed that there was no correlation between HYAL1 and HA in healthy control group，benign lesion group and colorectal cancer group（r values were 0.147，0.072 and -0.029，P>0.05）. The areas under curves（AUC） of HYAL1，HA，CEA and CA19-9 in the diagnosis of colorectal cancer were 0.899，0.723，0.717 and 0.669，respectively. The AUC of HYAL1 combined with CEA and CA19-9 were 0.917，0.905 and 0.916，respectively. The AUC of HYAL1，CEA and CA19-9 single and combined determinations were 0.860，0.686，0.685 and 0.885 in the diagnosis of early stage（TNM stage Ⅰ to Ⅱ） colorectal cancer. Serum HYAL1 levels were related with TNM stage，lymph metastasis，vascular invasion and nerve invasion in patients with colorectal cancer（P<0.001）. The levels of serum HYAL1，HA and CEA in patients with colorectal cancer after surgery were lower than those before surgery（P<0.01），and the decrease of HYAL1 was the most obvious. There was no statistical significance in serum CA19-9 before and after surgery（P>0.05）. Conclusions Serum HYAL1 may be used as a serological indicator for the auxiliary diagnosis of early colorectal cancer and efficacy monitoring.

Key words: Hyaluronidase 1, Hyaluronan, Colorectal cancer, Auxiliary diagnosis, Therapeutic efficacy monitoring

CLC Number:

R446.1

ZHANG Guoliang, LIU Yiwen, HE Yiqing, XU Jing, YANG Cuixia, GAO Feng, LIU Hua. Roles of serum HYAL1 and HA in the auxiliary diagnosis and therapeutic monitoring of colorectal cancer[J]. Laboratory Medicine, 2023, 38(4): 313-319.

Figures/Tables 8

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组别	例数	HYAL1/（pg/mL）	HA/（ng/mL）
正常对照组	51	880.80±453.60	55.32±10.38
良性疾病组	46	1 530.00±698.80^**	61.35±18.13
炎症组	15	1 927.00±593.40^*	57.81±13.55
腺瘤组	18	1 526.00±606.00^*	64.72±21.27
息肉组	13	1 077.00±693.00	60.77±18.56
结直肠癌组	75	2 148.00±930.90^**#	68.26±19.32^**
结肠癌组	41	2 067.00±956.30^**#	68.62±17.15^**
直肠癌组	34	2 246.00±852.30^**#	67.83±21.91^**

组别	例数	HYAL1/（pg/mL）	HA/（ng/mL）
正常对照组	51	880.80±453.60	55.32±10.38
良性疾病组	46	1 530.00±698.80^**	61.35±18.13
炎症组	15	1 927.00±593.40^*	57.81±13.55
腺瘤组	18	1 526.00±606.00^*	64.72±21.27
息肉组	13	1 077.00±693.00	60.77±18.56
结直肠癌组	75	2 148.00±930.90^**#	68.26±19.32^**
结肠癌组	41	2 067.00±956.30^**#	68.62±17.15^**
直肠癌组	34	2 246.00±852.30^**#	67.83±21.91^**

指标	AUC（95% CI^①）	最佳临界值	敏感性/%	特异性/%	漏诊率/%	误诊率/%	P值
HYAL1	0.899（0.846~0.951）	1 483.00 pg/mL	77.33	90.20	22.67	9.80	<0.000 1
HA	0.723（0.636~0.811）	64.91 ng/mL	56.00	84.31	44.00	15.69	<0.000 1
CEA	0.717（0.629~0.806）	3.13 ng/mL	57.33	86.27	42.67	13.73	<0.000 1
CA19-9	0.669（0.572~0.766）	12.83 U/ mL	52.00	88.24	48.00	11.76	0.001 3
HYAL1+CEA	0.917（0.870~0.964）	0.73	76.00	96.10	24.00	3.90	<0.000 1
HYAL1+CA19-9	0.905（0.854~0.957）	0.58	82.70	88.20	17.30	11.80	<0.000 1
HYAL1+CEA+CA19-9	0.916（0.868~0.964）	0.78	74.70	98.00	25.30	2.00	<0.000 1

指标	AUC（95% CI^①）	最佳临界值	敏感性/%	特异性/%	漏诊率/%	误诊率/%	P值
HYAL1	0.899（0.846~0.951）	1 483.00 pg/mL	77.33	90.20	22.67	9.80	<0.000 1
HA	0.723（0.636~0.811）	64.91 ng/mL	56.00	84.31	44.00	15.69	<0.000 1
CEA	0.717（0.629~0.806）	3.13 ng/mL	57.33	86.27	42.67	13.73	<0.000 1
CA19-9	0.669（0.572~0.766）	12.83 U/ mL	52.00	88.24	48.00	11.76	0.001 3
HYAL1+CEA	0.917（0.870~0.964）	0.73	76.00	96.10	24.00	3.90	<0.000 1
HYAL1+CA19-9	0.905（0.854~0.957）	0.58	82.70	88.20	17.30	11.80	<0.000 1
HYAL1+CEA+CA19-9	0.916（0.868~0.964）	0.78	74.70	98.00	25.30	2.00	<0.000 1

指标	AUC（95%CI）	最佳临界值	敏感性/%	特异性/%	P值
HYAL1	0.860（0.786~0.934）	1 787.00 pg/mL	69.57	90.20	<0.000 1
CEA	0.686（0.576~0.796）	3.13 ng/mL	56.52	84.31	0.001 6
CA19-9	0.685（0.566~0.804）	16.30 U/ mL	45.65	100.00	0.001 7
HYAL1 + CEA + CA19-9	0.885（0.817~0.953）	0.74	65.22	98.04	<0.000 1