Influence of specimen state on the accuracy of plasma Epstein-Barr virus DNA determination results

doi:10.3969/j.issn.1673-8640.2022.07.003

Abstract

Abstract:

Objective To investigate the expression of Epstein-Barr virus（EBV）DNA in hemolysis and lipoidemia of plasma samples. Methods A total of 40 plasma positive samples with confirmed EBV DNA were collected and divided into 2 groups randomly（20 cases for each group）. The EBV DNA positive samples were mixed with sterilized distilled water in a 1∶1 ratio as control samples. The levels of EBV DNA in simulated hemolysis（hemoglobin concentration 60.50,30.25 and 15.13 g/L） and simulated lipoidemia（triglyceride concentration 6.36,4.24 and 2.12 mmol/L） were determined by fluorescence quantitative polymerase chain reaction（PCR）,and statistical analysis was performed. Results When the hemoglobin concentrations were 60.50 and 30.25 g/L,the PCR results of EBV DNA positive plasma hemolysis samples and control samples showed statistical significance（P<0.05）. When the hemoglobin concentration was 15.13 g/L,there was no statistical significance in the PCR results between EBV DNA positive plasma hemolysis samples and control samples（P>0.05）. There was no statistical significance in the PCR results between lipoidemia samples and control samples（P>0.05）. Conclusions Hemolysis samples with hemoglobin concentration ≤15.13 g/L and lipoidemia samples with triglyceride concentration ≤6.36 mmol/L had no effect on the determination of EBV DNA plasma samples.

Key words: Epstein-Barr virus DNA, Fluorescence quantitative polymerase chain reaction, Hemolysis, Lipoidemia

CLC Number:

R373.1

WANG Xiaoyu, SEMIRE Ainiwaer, LI Shijun. Influence of specimen state on the accuracy of plasma Epstein-Barr virus DNA determination results[J]. Laboratory Medicine, 2022, 37(7): 615-617.

Figures/Tables 2

References 9

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样本类型	血红蛋白
样本类型	15.13 g/L	30.25 g/L	60.50 g/L
对照样本	9 567.17±3 363.79	9 567.17±3 363.79	9 567.17±3 363.79
溶血样本	7 193.91±2 239.33	7 634.28±2 617.17	6 525.52±2 072.07
t值	1.896	2.295	2.263
P值	0.073	0.033	0.036

样本类型	血红蛋白
样本类型	15.13 g/L	30.25 g/L	60.50 g/L
对照样本	9 567.17±3 363.79	9 567.17±3 363.79	9 567.17±3 363.79
溶血样本	7 193.91±2 239.33	7 634.28±2 617.17	6 525.52±2 072.07
t值	1.896	2.295	2.263
P值	0.073	0.033	0.036

样本类型	三酰甘油
样本类型	6.36 mmol/L	4.24 mmol/L	2.12 mmol/L
对照样本	8 668.50±3 622.02	8 668.50±3 622.02	8 668.50±3 622.02
脂血样本	8 528.10±3 670.38	8 482.60±3 515.42	8 433.45±3 347.40
t值	1.180	1.448	0.760
P值	0.252	0.164	0.456

样本类型	三酰甘油
样本类型	6.36 mmol/L	4.24 mmol/L	2.12 mmol/L
对照样本	8 668.50±3 622.02	8 668.50±3 622.02	8 668.50±3 622.02
脂血样本	8 528.10±3 670.38	8 482.60±3 515.42	8 433.45±3 347.40
t值	1.180	1.448	0.760
P值	0.252	0.164	0.456

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