Abstract:

Objective To evaluate the role of immature platelet fraction（IPF%） determination by Sysmex XN automated hematology analyzer for thrombocytopenia. Methods The platelet（PLT） counts and IPF% of 200 patients with thrombocytopenia [45 patients with primary immune thrombocytopenia（ITP）,40 patients with aplastic anemia（AA）,60 patients undergoing chemotherapy（CT） and 55 patients with chronic liver diseases（CLD）] and 50 healthy subjects（healthy control group） were determined by Sysmex XN automated hematology analyzer. The difference of IPF% was analyzed by GraphPad Prism 6 software,and receiver operating characteristic（ROC） curve analysis was utilized to demonstrate the diagnostic efficiency of IPF% in the assessment of thrombocytopenia. Results The PLT counts of ITP,AA,CT and CLD groups were lower than that of healthy control group（P<0.05）. However,there was no statistical significance between ITP and AA groups and between CT and CLD groups（P>0.05）. IPF% of ITP group was higher than those of healthy control,AA,CT and CLD groups（P<0.05）,while there was no statistical significance between AA,CT and CLD groups and healthy control group（P>0.05）. When the cut-off value of IPF% was 5.8%,the sensitivity was 82.2%,the specificity was 86.8%,and the area under curve（AUC） was 0.92. Conclusions IPF% determination by Sysmex XN automated hematology analyzer plays a role for discriminating hypo- from hyper-proliferative thrombocytopenias.

Key words: Immature platelet fraction, Thrombocytopenic disorder, Automated hematology analyzer