Abstract:

Objective To investigate the influence of different placed times of blood specimens on some biochemical indicators of cardiovascular disease related determination. Methods A total of 80 patients undergoing routine biochemical determination were enrolled,and every patient was extracted for 6 tubes of blood specimens. Serum of 1 tube was extracted by pipette and divided into 4 cryovials,which were determined immediately,after 2-8 ℃ refrigerated overnight,after -20 ℃ refrigerated for 24 h and after -20 ℃ refrigerated for 72 h. The other 3 tubes were determined after being placed at room temperature for 1,3 and 6 h,while the other 2 tubes were placed at room temperature for 6 h,and were determined after -20 ℃ refrigerated for 24 and 72 h. Ischemia modified albumin（IMA）,lactate dehydrogenase（LDH）,aspartate aminotransferase isoenzyme（m-AST） and free fatty acid（FFA） were determined. The difference of different placed times of blood specimens on some biochemical indicators of cardiovascular disease related determination was evaluated. Results Compared to the results of those determined immediately,LDH and FFA increased,while m-AST and IMA decreased in those determined after being placed at room temperature for 1 h（P>0.05）. As for those determined after 2-8 ℃ refrigerated overnight and those determined after being placed at room temperature for 3 and 6 h,LDH and FFA increased（P<0.05）,m-AST and IMA decreased（P<0.05）,and LDH and FFA for 6 h were higher than those for 3 h（P<0.01）. The results of those determined after -20 ℃ refrigerated for 24 and 72 h had no statistical significance compared with those determined immediately（P>0.05）. The results of those determined after being placed at room temperature for 6 h and determined after -20 ℃ refrigerated for 24 and 72 h were higher than those determined immediately（P<0.01）,and m-AST and IMA decreased（P<0.01）. Conclusions Long placed times of blood specimens have influence on some biochemical indicators of cardiovascular disease related determination.

Key words: Blood specimen, Placed time, Biochemical indicator