Abstract: Objective To investigate 2 cases of amikacin application in validation for ethylene diamine tetracetic acid (EDTA)-dependent pseudo thrombocytopenia. Methods Two cases of EDTA-dependent pseudo thrombocytopenia were collected, and their EDTA-K₂ anticoagulant blood were collected and added amikacin at different time periods.The blood samples were determined for hematology analysis and blood smears. Results For the 2 patients with or without amikacin, the platelet count increased gradually with time, and the histogram disappeared with time. Adding amikacin would obtain more reliable results. Conclusions Amikacin can not immediately dissociate the platelet aggregation in the 2 cases, however, with time, the slow dissociation of platelet achieves a relatively stable state for 4 h. Amikacin is added within 1 h is preferable. The relatively common EDTA anticoagulation can effectively accelerate the dissociation process, in order to obtain the desired results.

Key words: Pseudo thrombocytopenia, Amikacin