Abstract: Objective To fit 4 parameter logistic calibration of DXI 800 chemiluminescence analyzer by Origin software and compare the datum difference between Origin and DXI 800, and to investigate the calculation possibility of unknown results of DXI 800 by Origin software. Methods The calibration results of testosterone, total thyroxine, estradiol, troponin I, thyrotropic hormone, carcino-embryonic antigen and progesterone were collected and fit by Origin software, and the calculation results with the original results of DXI 800 system were analyzed comparatively. Results The calculation results of Origin software were very close to the original results of DXI 800, and mean bias was from -0.85% to 1.68%. Conclusions Origin software is suitable for the calculation of unknown results of DXI 800 chemiluminescence analyzer.

Key words: Four parameter logistic calibration, Origin software, Fit