Abstract: Objective To investigate the influence of anti-sperm antibodies (AsAb) on sperm morphology. Methods A total of 51 AsAb positive patients,40 control patients and 47 healthy controls were enrolled. The sperm morphology was evaluated according to World Health Organization (WHO) sperm morphology classification standards by Diff-Quik staining method. The abnormal sperm index (TZI) and sperm deformity index (SDI) were calculated. Results In the AsAb positive patients, the percentages of abnormal morphology (%), cone-shaped head (%), amorphous head (%) and middle neck thickening sperms (%) were 80.2?7.7, 6.3?5.5, 11.0?7.4 and 21.9?9.0, respectively. In the control patients, these percentages were 71.6?5.8, 4.2?4.5, 11.1?4.8 and 16.0?6.9, respectively. In the healthy controls, these percentages were 70.1?6.4, 3.5?4.6, 8.4?4.3 and 16.2?6.9, respectively. There were statistical significances between the 3 groups as to the above mentioned parameters (F values were 28.732, 3.867, 3.282 and 4.893, P<0.05 or P<0.01).The TZI and SDI were 1.306?0.123 and 1.057?0.180 in the AsAb positive patients, 1.255?0.096 and 0.901?0.119 in the control patients, and 1.257?0.098 and 0.882?0.121 in the healthy controls, respectively. There were statistical significances between the 3 groups (F values were 3.088 and 19.115, P<0.05 or P<0.01). Conclusions The positivity of AsAb is one of the reasons for the increasing of abnormal sperm morphology. Abnormal sperm morphology has mainly cone-shaped head, amorphous head and middle neck thickening sperms.

Key words: Anti-sperm antibody, Sperm morphology, Multiple sperm defect index