Influence of freeze-thaw treatment on determination results of thrombophilia in routine laboratory specimens

doi:10.3969/j.issn.1673-8640.2025.10.014

Abstract

Abstract:

Objective To analyze the influence of routine laboratory specimens subjected to freezing and thawing at -20 ℃ on the determination results of thrombophilia. Methods A total of 80 sodium citrate anticoagulant plasma specimens from the First Affiliated Hospital of Xi'an Jiaotong University were collected. The levels of antithrombin（AT），protein C（PC），lupus anticoagulant（LA）and protein S（PS） were determined at the immediate time point（0 d），immediately after thawing at -20 ℃ for 3 d（3 d） and 2 h（2 h） after thawing at 4 ℃. The differences in determination results at different time points were compared. Results The levels of AT，PC and LA remained stable within 2 h after thawing at -20 ℃ for 3 d，while the level of PS was significantly decreased，but the results remained stable within 2 h after thawing. Conclusions Freezing and thawing of sodium citrate anticoagulated plasma at -20 ℃ has an impact on the determination results of PS. Clinicians should pay attention to this situation when diagnosing thrombophilia.

Key words: Thrombophilia, Anticoagulant protein, Lupus anticoagulant, Plasma specimen, Freezing and thawing

CLC Number:

Q446.11

WANG Shuaili, LIU Ying, WANG Li, YUAN Li. Influence of freeze-thaw treatment on determination results of thrombophilia in routine laboratory specimens[J]. Laboratory Medicine, 2025, 40(10): 1010-1013.

Figures/Tables 3

References 17

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组别	AT/%	PC/%	PS/%	LA-S/s	LA-C/s	LA-ratio
0 d	70.80±26.83	66.03±24.88	79.18±31.93	35.15（32.55，55.58）	33.30（31.10，37.18）	1.02（0.96，1.51）
3 d	70.20±27.27	66.78±23.50	69.42±28.18^*	35.00（33.28，55.98）	34.45（32.83，38.33）	1.01（0.97，1.52）
2 h	70.13±26.83	66.47±23.21	69.09±28.20^*	36.75（34.33，55.28）	35.60（33.73，39.40）	1.03（0.96，1.49）
统计值	1.412	3.174	71.937	1.728	5.314	0.169
P值	0.255	0.079	<0.001	0.528	0.070	0.919

组别	AT/%	PC/%	PS/%	LA-S/s	LA-C/s	LA-ratio
0 d	70.80±26.83	66.03±24.88	79.18±31.93	35.15（32.55，55.58）	33.30（31.10，37.18）	1.02（0.96，1.51）
3 d	70.20±27.27	66.78±23.50	69.42±28.18^*	35.00（33.28，55.98）	34.45（32.83，38.33）	1.01（0.97，1.52）
2 h	70.13±26.83	66.47±23.21	69.09±28.20^*	36.75（34.33，55.28）	35.60（33.73，39.40）	1.03（0.96，1.49）
统计值	1.412	3.174	71.937	1.728	5.314	0.169
P值	0.255	0.079	<0.001	0.528	0.070	0.919

项目	AT	PC	PS	LA-S	LA-C	LA-ratio
3 d	-0.85	1.14	-12.33	-0.43	3.45	-0.98
2 h	-0.95	0.67	-12.74	4.55	6.91	0.98
1/2允许总误差	10.00	7.5	12.50	7.50^①	7.50^①	不适用

项目	AT	PC	PS	LA-S	LA-C	LA-ratio
3 d	-0.85	1.14	-12.33	-0.43	3.45	-0.98
2 h	-0.95	0.67	-12.74	4.55	6.91	0.98
1/2允许总误差	10.00	7.5	12.50	7.50^①	7.50^①	不适用