Correlation of serum miR-21 and miR-155 levels and vaginal microecological changes in patients with cervical cancer

doi:10.3969/j.issn.1673-8640.2025.03.013

Abstract

Abstract:

Objective To investigate the correlation of serum miR-21 and miR-155 levels and vaginal microecological changes in patients with cervical cancer （CC）. Methods From January 2020 to December 2021，68 patients with CC from Suzhou Municipal Hospital（North District）were enrolled as CC group，60 patients with cervical intraepithelial neoplasia （CIN） were enrolled as CIN group，and 60 healthy subjects were enrolled as control group. Quantitative real-time polymerase chain reaction （PCR） was used to determine the levels of serum miR-21 and miR-155. Smear microscopy，Gram staining smears and vaginal microbial functional assay were used to determine vaginal microecological indicators. Pearson correlation was used to analyze the correlation of serum miR-21 and miR-155 in CC group. Spearman correlation was used to analyze the correlation between serum miR-21，miR-155 and vaginal microecological indicators. Results Compared with control group，serum miR-21 and miR-155 levels were higher in CIN and CC groups（P<0.05）. The levels of serum miR-21 and miR-155 and the positive rate of human papillomavirus（HPV）16/18 in CC group were higher than those in CIN group（P<0.05）. The proportions of vaginal flora density（grade 1 or 4），aerobic vaginitis（AV）and bacterial vaginosis（BV）in CC group were higher than those in CIN group（P<0.05），and there was no statistical significance in the proportions of vaginal flora diversity （grade 1 or 4），cleanliness（grade 3-4），pH>4.5，trichomonal vaginitis（TV），positive H₂O₂ and positive leukocyte esterase between CIN and CC groups（P>0.05）. Serum miR-21 and miR-155 levels in CC group were positively correlated（r=0.524，P<0.001）. Serum miR-21 and miR-155 levels were positively correlated with HPV16/18 positive rate，vaginal flora density（grade 1 or 4），AV and BV（P<0.001）. Conclusions The expressions of serum miR-21 and miR-155 in CC patients are up-regulated，and they have a certain correlation with HPV16/18 infection and vaginal microecological changes.

Key words: MiR-21, MiR-155, Vaginal microecology, Cervical cancer

CLC Number:

R446.7

ZHOU Ying, XU Ying, QIU Jianping. Correlation of serum miR-21 and miR-155 levels and vaginal microecological changes in patients with cervical cancer[J]. Laboratory Medicine, 2025, 40(3): 278-282.

Figures/Tables 5

References 25

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组别	例数	年龄/岁	体重指数/（kg·m^-2）	孕次/次	产次/次	宫颈长度/cm
CC组	68	48.25±7.26	23.04±2.21	2.50±0.45	1.75±0.35	4.26±0.59
CIN组	60	48.80±7.18	22.76±2.29	2.60±0.41	1.85±0.39	4.13±0.68
对照组	60	48.45±7.35	22.55±2.41	2.60±0.42	1.70±0.45	4.02±0.75
统计值		0.093	0.733	1.185	2.230	2.041
P值		0.912	0.482	0.308	0.111	0.133

组别	例数	年龄/岁	体重指数/（kg·m^-2）	孕次/次	产次/次	宫颈长度/cm
CC组	68	48.25±7.26	23.04±2.21	2.50±0.45	1.75±0.35	4.26±0.59
CIN组	60	48.80±7.18	22.76±2.29	2.60±0.41	1.85±0.39	4.13±0.68
对照组	60	48.45±7.35	22.55±2.41	2.60±0.42	1.70±0.45	4.02±0.75
统计值		0.093	0.733	1.185	2.230	2.041
P值		0.912	0.482	0.308	0.111	0.133

组别	例数	miR-21	miR-155	HPV16/18型阳性率/[%（例）]
CC组	68	1.87±0.49^*#	1.72±0.42^*#	73.53（50）
CIN组	60	1.03±0.08^*	1.02±0.09^*	35.00（21）
对照组	60	0.62±0.11	0.71±0.13
统计值		282.384	240.839	19.157
P值		<0.001	<0.001	<0.001

组别	例数	miR-21	miR-155	HPV16/18型阳性率/[%（例）]
CC组	68	1.87±0.49^*#	1.72±0.42^*#	73.53（50）
CIN组	60	1.03±0.08^*	1.02±0.09^*	35.00（21）
对照组	60	0.62±0.11	0.71±0.13
统计值		282.384	240.839	19.157
P值		<0.001	<0.001	<0.001

组别	例数	阴道菌群密集度（1级或4级）/[例（%）]	阴道菌群多样性（1级或4级）/[例（%）]	清洁度（3~4度）/ [例（%）]	AV/ [例（%）]	BV/ [例（%）]	TV/ [例（%）]	pH值>4.5/ [例（%）]	H₂O₂阳性/[例（%）]	白细胞酯酶阳性/ [例（%）]
CC组	68	54 （79.41）	32 （47.06）	52（76.47）	46（67.65）	50（73.53）	4 （5.88）	49（72.06）	45（66.18）	37（54.41）
CIN组	60	35 （58.33）	25 （41.67）	46（76.67）	28（46.67）	33（55.00）	2 （3.33）	41（68.33）	39（65.00）	30（50.00）
统计值		6.685	0.375	0.001	5.753	4.801	0.069	0.212	0.020	0.249
P值		0.010	0.540	0.979	0.016	0.028	0.793	0.645	0.889	0.618