Discussion on the application of high-throughput metagenomic sequencing of pathogens in the diagnosis of pulmonary infections in adults

doi:10.3969/j.issn.1673-8640.2026.03.002

Abstract

Abstract:

In recent years，metagenomic next-generation sequencing （mNGS）technology has developed rapidly in the research and application of infectious diseases，especially in pulmonary infections. It has played a positive role in the diagnosis of infections caused by rare pathogens that are difficult to determine by traditional methods. However，the mNGS technology still has limitations that need to be overcome. The positive threshold standards are not unified，and there are misjudgements in the determination of Candida，Mycobacterium tuberculosis and Pneumocystis jiroveci. This review focuses on the research direction and development of mNGS technology from the perspective of microbial diagnosis management.

Key words: Metagenomic next-generation sequencing, Pulmonary infection, Microbial diagnosis management

CLC Number:

R446.7

HE Lixian. Discussion on the application of high-throughput metagenomic sequencing of pathogens in the diagnosis of pulmonary infections in adults[J]. Laboratory Medicine, 2026, 41(3): 217-222.

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