Analysis on the correlation of CD40-1C/T gene polymorphism with children asthma

Abstract

Abstract:

Objective　To investigate the distribution of CD40 gene 5′untranslated region-1 site C/T gene polymorphism，and to study its relationship with the risk of asthma in local asthma children. Methods　A total of 134 asthma children were enrolled，and 113 healthy children from healthy examination subjects were as control group in asthma and contol groups. Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）was used to determine the genotype and the allele frequencies of CD40-1C/T gene. The serum total level of IgE was determined by immunonephelometry. The relationship of CD40-1C/T gene polymorphism with the risk of asthma and IgE level was analyzed. Results　The distributions of the C and T allele frequencies between asthma and control groups had no statistical significance （P＞0.05). The distribution difference of CC and CT+TT genotype frequencies was statistically significant （P＜0.05). The risk of CC genotype was 1.75 times higher than that of CT+TT genotype [95%confidence interval （CI）：1.01-3.04].There were significant differences in the level of IgE between CC genotype and CT or TT genotype in asthma group （P＜0.05). Conclusions　CC genotype of CD40 gene 5′untranslated region -1 site might be associated with the development of asthma in local asthma children.

Key words: CD40, Asthma, Gene polymorphism

DU Jiangang 1，XU Hongxing 1，JIE Qiongfei 1，ZHONG Qiao 1，WU Yuanjian 1，XU Jicheng 2. Analysis on the correlation of CD40-1C/T gene polymorphism with children asthma[J]. , 2013, 28(1): 17-20.

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