The inhibition of cell proliferation and invasion through miR-34a regulating YY1 in human renal carcinoma cell

›› 2012, Vol. 27 ›› Issue (8): 635-640.

The inhibition of cell proliferation and invasion through miR-34a regulating YY1 in human renal carcinoma cell

Department of Clinical Laboratory，the Tenth People′s Hospital of Tongji University，Shanghai 200072，China

Received:2012-03-09 Online:2012-08-30 Published:2012-08-08
Contact: zhi LI

Abstract

Abstract: Objective　To investigate miR-34a expression in renal carcinoma cell and the inhibitory effect and regulating mechanism of miR-34a on the proliferation and invasion in human renal carcinoma ACHN cells. Methods　The expressions of miR-34a in cancer and pericancerous tissues were detected by real-time polymerase chain reaction（PCR）. ACHN cells were transfected with miR-34a mimics and there set blank control group, negative control group and miR-34a mimics group. The expression of miR-34a was measured by real-time PCR 24 h after transfection. The cell proliferation and cell cycle were determined by 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide （MTT） and flow cytometry. The invasive ability was examined by Transwell and Matrigel invasive assays. The mRNA and protein levels of YY1 were detected by real-time PCR and Western blot. Results　The relative expression of miR-34a in cancer tissues （1.06±0.67） was significantly lower than that in pericancerous tissues （1.62±0.83, P＜0.01）. After transfection 24 h， an increase expression of miR-34a was noted in miR-34a mimics group （157.04±13.01） comparing with negative control group（P＜0.01）. Overexpression of miR-34a significantly inhibited the cell proliferation（P＜0.01）. The cell cycle was arrested at G0/G1 phases.Transwell and Matrigel invasive assays showed that the invasive ability of cells was significantly suppressed after transfection（P＜0.01）. YY1 gene had no mRNA expressions after transfection （P＞0.05）. Conclusions　The expression of miR-34a is low in renal carcinoma，and it may be correlated with tumorigenesis，partially through regulating YY1.

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WANG Mingli;LI Zhi;XU Jinyu;WENG Wenhao;XU Shanshan. The inhibition of cell proliferation and invasion through miR-34a regulating YY1 in human renal carcinoma cell[J]. , 2012, 27(8): 635-640.

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