Abstract: Objective　To evaluate the influence of variant hemoglobin and hemoglobin derivative carbamino hemoglobin on the detection of glycosylated hemoglobin （HbA1c） by ion exchange-high performance liquid chromatography（HPLC） and enzymatic method.  Methods　The normal hemoglobin structure diabetes mellitus patients′ samples without uremia，the fetal hemoglobin（HbF） samples and the concentration of HbA1c in patients with uremia were detected by the 2 Methods simultaneously.  Results　There was no significant difference between the 2 Methods for the HbA1c concentration in the normal hemoglobin structure diabetes mellitus samples without uremia（P>0.05）. For the ion exchange-HPLC， when HbF < 8.75%， there was no significant difference between the measured results and the expected value. When HbF was 8.8%-23.0%， the results were significantly lower than the theoretical concentration of HbA1c. When HbF was 35.0%-70.0%， the concentration of HbA1c can not be detected. For the carbamino hemoglobin， the HbA1c concentration in patients with uremia by ion exchange-HPLC was significantly higher than that by enzymatic method. Enzymatic method of HbA1c could not be interfered by HbF and carbamino hemoglobin.  Conclusions　The determination of HbA1c is influenced by HbF and carbamino hemoglobin by ion exchange-HPLC. There is no interference by enzymatic method.

Key words: Carbamino hemoglobin, Variant hemoglobin, Ion exchange-high performance liquid chromatography, Enzymatic method, Glycosylated hemoglobin