Research on virulence gene of multi-source methicillin-resistant Staphylococcus aureus

Abstract

Abstract: Objective　To investigate the infection status of clinical methicillin-resistant Staphylococcus aureus（MRSA），and analyze the distribution of Staphylococcus aureus（SA） virulence gene in clinical detection of MRSA and methicillin-sensitive Staphylococcus aureus（MSSA）. Methods　A total of 60 clinical MRSA isolates and 54 clinical MSSA isolates were collected from January 2009 to June 2011 in Quzhou People′s Hospital. Polymerase chain reaction（PCR） was used to detect virulence gene panton-valentine leukocidin（pvl），staphylococcal enterotoxin C（sec），staphylococcal enterotoxin H（seh），hemolysin A （Hla），hemolysin B（Hlb），clumping factor A（clfA），clumping factor B（clfB），fibronection-binding protein A（fnb A） and fibronection-binding protein protein B（fnbB）of these clinical SA isolates. Results　A majority of 60 MRSA isolates distributed in Neurosurgery，General Surgery， Paediatrics and Orthopaedics Departments. The 17 isolates of 60 MRSA were considered as community-associated MRSA（CA-MRSA）， and 43 isolates of 60 MRSA were hospital-associated MRSA（HA-MRSA）. In contrast to MSSA， the positive detection rate of pvl in MRSA was higher， but the positive detection rates of clfA，fnbA，sec and seh were lower. The positive rates of pvl and fnbA in CA-MRSA were higher than those in HA-MRSA. Conclusions　The virulence of MSSA isolates is probably higher than that of MRSA. The virulence of CA-MRSA isolates is higher than that of HA-MRSA.The different distribution of the virulence genes of these clinical SA isolates may be associated with the site of infection.

ZHU Jin;LU Jun;YU Xu-Liang;BAI Yong-Feng. Research on virulence gene of multi-source methicillin-resistant Staphylococcus aureus[J]. , 2012, 27(6): 475-478.

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Research on virulence gene of multi-source methicillin-resistant Staphylococcus aureus

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