Epidemiology and drug-resistance analysis of nosocomial infection of Candida glabrata

Abstract

Abstract: Objective　To investigate genotypes and drug susceptibility of Candida glabrata isolated from Renji Hospital in order to study its drug-resistance and epidemic situation， and investigate their correlation. Methods　Multilocus sequence typing （MLST）technique was introduced to identify sequences of 6 housekeeping genes from 34 isolates of Candida glabrata. The results were compared with sequence information in MLST databases by Clustalx software to determine a strain allelic profile and sequence type（ST）. By drawing the phylogenetic tree by Clustalx software， the isolates were classified into various clonal complexes by eBURST program to compare the genetic relationship. ATB FUNGUS semi-automatic system was used to test the drug susceptibility. Ridit analysis method was used to analyze the correlation between the genotypes and drug susceptibility. Results　The 34 isolates belonged to 6 clone sequences. ST-7 had the most proportion of 79.4%（27/34）. There were 3 isolates belonged to ST-10，accounting for 8.8%（3/34）， and the remaining 4 isolates belonged to ST-3，ST-15， ST-43 and ST-55，respectively. A total of 34 isolates of Candida glabrata were 100.0% sensitive to fluorocytosine and amphotericin，while the sensitive rates of voriconazole and fluconazole were 97.1% and 91.2%，respectively.The effect of itraconazole was poor with the sensitive rate of 11.8% As ST-7 for the standard group，the Ridit values in the group of ST-10 and other types of ST were included the mean Ridit value of the standard group in fluconazole，voriconazole and itraconazole. Conclusions　ST-7 is the dominant genotype in Renji Hospital. No correlation between the Candida glabrata genotype and its drug susceptibility is found.

ZHENG Bing;YAO Dong-Ting;YING Chun-Mei;WANG Ya-Ping;ZHANG Hao-Min;YANG Jun. Epidemiology and drug-resistance analysis of nosocomial infection of Candida glabrata[J]. , 2012, 27(6): 461-466.

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